Enhanced cell adhesion to silicone implant material through plasma surface modification

被引：0
作者：
J. Hauser
J. Zietlow
M. Köller
S. A. Esenwein
H. Halfmann
P. Awakowicz
H. U. Steinau
机构：
[1] BG Trauma Center Bergmannsheil,Department of Plastic Surgery
[2] University Hospital,Department of Surgical Research
[3] BG Trauma Center Bergmannsheil,Institute for Plasma Technology
[4] University Hospital,Department of Surgery
[5] Ruhr-University,Department of Plastic Surgery
[6] BG Trauma Center Bergmannsheil,undefined
[7] University Hospital,undefined
[8] BG-Kliniken Bergmannsheil,undefined
[9] University Hospital,undefined
来源：
Journal of Materials Science: Materials in Medicine | 2009年 / 20卷
关键词：
Plasma Treatment; Surface Free Energy; Silicone Surface; Breast Implant; Capsular Contracture;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Silicone implant material is widely used in the field of plastic surgery. Despite its benefits the lack of biocompatibility this material still represents a major problem. Due to the surface characteristics of silicone, protein adsorption and cell adhesion on this polymeric material is rather low. The aim of this study was to create a stable collagen I surface coating on silicone implants via glow-discharge plasma treatment in order to enhance cell affinity and biocompatibility of the material. Non-plasma treated, collagen coated and conventional silicone samples (non-plasma treated, non-coated) served as controls. After plasma treatment the change of surface free energy was evaluated by drop-shape analysis. The quality of the collagen coating was analysed by electron microscopy and Time-Of-Flight Secondary Ion Mass Spectrometry. For biocompatibility tests mouse fibroblasts 3T3 were cultivated on the different silicone surfaces and stained with calcein-AM and propidium iodine to evaluate cell viability and adherence. Analysis of the different surfaces revealed a significant increase in surface free energy after plasma pre-treatment. As a consequence, collagen coating could only be achieved on the plasma activated silicone samples. The in vitro tests showed that the collagen coating led to a significant increase in cell adhesion and cell viability.
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页码：2541 / 2548
页数：7
相关论文
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