Determination of Sulfonamide Residues in Chicken Liver Using High-Performance Liquid Chromatography

A novel high-performance liquid chromatography method was adopted for determination of sulfacetamide, sulfathiazole, sulfamethazine, and sulfamethoxypyridazine residues in chicken liver based on the coupling of dispersive solid-phase extraction (DSPE) and dispersive liquid-liquid microextraction (DLLME). In the DSPE-DLLME method, chicken livers were firstly extracted with 50 mM phosphoric acid:MeCN (30:70), then cleaned up with a solid adsorbent. One milliliter of the resulting liquid supernatant extract and 650 μL of 1,2-dichloroethane were then added to a centrifuge tube containing 10 mL water. The analytes were extracted under optimized pretreatment conditions. Signal linearity over the concentration range of 0.006–4.00 mg/kg was observed (regression coefficients, 0.9988–0.9992), and limits of detection were in the range of 0.0004–0.0084 mg/kg. The present method was applied to the determination of the sulfonamides in chicken liver samples. The spiked recovery values ranged from 70.2 to 91.4 % and the relative standard deviations were less than 5.35 % (n = 6).