Nutritional Requirements to Improve Delta-Endotoxins Production of Bacillus thuringiensis var. kurstaki Using Mixed Designs Modelling

被引：2

作者：

Ennouri K. ^{[1
]}

Ben Hassen H. ^{[2
]}

Zouari N. ^{[3
]}

机构：

[1] Team of Biopesticides, Laboratory of Protection and Improvement of Plants, Centre of Biotechnology of Sfax, Sfax

[2] Laboratory of Physics, Mathematics and Applications, Sfax University, Sfax

[3] Biological & Environmental Sciences Department, College of Arts and Sciences, Qatar University, Doha

来源：

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2017年 / 87卷 / 2期

关键词：

Bacillus thuringiensis kurstaki; Culture medium; Insecticidal toxins; Mixed Plackett–Burman designs; Multiple linear regression;

D O I：

10.1007/s40011-015-0620-z

中图分类号：

学科分类号：

摘要：

Bacillus thuringiensiskurstaki is a soil bacterium that produces insecticidal toxins called delta-endotoxins. In order to increase the toxic crystal concentrations in a low-cost culture medium and thus improve the biopesticide quality to control insect pests, the Plackett–Burman screening method was applied. It was shown a tool to evaluate the significance of the selected seven factors (KH2PO4, K2HPO4, MgSO4, MnSO4, FeSO4, soybean meal, starch) which are necessary to the production of the delta-endotoxins. This was performed into two different shake flasks (250 and 500 ml). The main factors that affected the production of delta-endotoxins are shown to be soybean meal, starch, and FeSO4 in 250 ml culture flasks. In 500 ml culture flasks, soybean meal and FeSO4 are the principal factors influencing the delta-endotoxin production. The multiple linear regression, a method applied as the merging dataset of the two Plackett–Burman designs, established that soybean meal and starch are the factors positively affecting the production of delta-endotoxins, in contrast to FeSO4. Furthermore, the available oxygen in culture flasks showed no significant negative effect on delta-endotoxin production. This study revealed that mixed method designs were useful to identify the significance and the effect of hidden culture parameters. © 2015, The National Academy of Sciences, India.

引用

页码：307 / 314

页数：7

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