Isolation and molecular characterization of a cryptic plasmid from Bifidobacterium longum

被引：0
作者：
Young-Seo Park
Ki-Hwan Kim
Ju Hui Park
In-Kyung Oh
Sung-Sik Yoon
机构：
[1] Kyungwon University,Department of Food Science and Biotechnology
[2] Kyungwon University,Type Culture Bank of Korea
[3] Yonsei University,Department of Biological Resources and Technology
[4] Yonsei University,Institute of Functional Biomaterials and Biotechnology
来源：
Biotechnology Letters | 2008年 / 30卷
关键词：
Mobilization; Protein plasmid isolation; Replication protein;
D O I：
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中图分类号：
学科分类号：
摘要：
An isolate from the fecal samples of children was identified as Bifidobacterium longum. A plasmid isolated from it pBIFA24 was 4,892 bp with three open reading frames, ORFI, ORFII, and ORFIII. ORFI encoded a replication protein involved in a rolling-circle replication mechanism, and three sets of tandem repeat sequences featuring iteron structure were identified. Secondary structure prediction analysis of ORFII suggested it was a transmembrane protein. ORFIII showed high amino acid sequence identity with some mobilization proteins and contained an oriT sequence.
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页码：145 / 151
页数：6
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