CARD15/NOD2 Is Not a Predisposing Factor for Necrotizing Enterocolitis

被引：0
作者：
Habib Zouali
Arnaud Bonnard
Pascal De Lagausie
Caroline Farnoux
Yves Aigrain
Jean-Pierre Cezard
Michel Peuchmaur
Jean-Pierre Hugot
Dominique Berrebi
机构：
[1] Université,Fondation Jean Dausset
[2] Université,CEPH
[3] Université,Service de Chirurgie Pédiatrique
[4] Université,Service de Néonatologie
[5] Université,Service de Gastroentérologie Pédiatrique
[6] Université,Service d'Anatomie et Cytologie Pathologiques
[7] Université,INSERM U458, Hôpital Robert Debré
[8] Fondation Jean Dausset-CEPH 27,EA3102
来源：
Digestive Diseases and Sciences | 2005年 / 50卷
关键词：
CARD15/NOD2; innate immunity; necrotizing enterocolitis; inflammation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Multiple factors are incriminated in the etiopathogeny of necrotizing enterocolitis (NEC) in premature infants, including oral feeding, vascular abnormalities, increase in pro-inflammatory cytokines, and inappropriate response of the intestinal barrier to bacterial microflora. CARD15/NOD2 is a gene recently recognized as important in the innate response to gut flora and is involved in Crohn's disease susceptibility. We thus tested its putative role in NEC. Ten children (seven boys and three girls) suffering from NEC who were admitted to Robert Debré hospital between 1999 and 2002 were retrospectively included in the study. Genetic screening of the 11 constant exons and the exon–intron junctions of CARD15/NOD2 by direct sequencing revealed no novel mutations of that gene in NEC patients. Furthermore, the three main mutations of CARD15/NOD2 (R702W, G908R, and 1007fs) associated with susceptibility to Crohn's disease were not found in these patients. Our results suggest that CARD15/NOD2 does not play a major role in genetic susceptibility to NEC.
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页码：1684 / 1687
页数：3
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  • [1] Hsueh W(2003)Neonatal necrotizing enterocolitis: clinical considerations and pathogenetic concepts Pediatr Dev Pathol 6 6-23
  • [2] Caplan MS(2003)Neonatal necrotizing enterocolitis: an overview Curr Opin Infect Dis 16 349-355
  • [3] Qu XW(1994)Neonatal necrotizing enterocolitis, a disease of the immature intestinal mucosal barrier Acta Paediatr (Suppl) 396 27-32
  • [4] Tan XD(2000)Inflammation in the developing human intestine: A possible pathophysiologic contribution to necrotizing enterocolitis Proc Natl Acad Sci USA 97 6043-6048
  • [5] De Plaen IG(2002)Pattern recognition receptors: doubling up for the innate immune response Cell 111 927-930
  • [6] Gonzalez-Crussi F(2005)LPS, TLR4 and infectious disease diversity Nat Rev Microbiol 3 36-46
  • [7] Kafetzis DA(2003)Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection J Biol Chem 278 8869-8872
  • [8] Skevaki C(2003)CARD15 gene overexpression in mononuclear and epithelial cells of the inflamed Crohn's disease colon Gut 52 840-846
  • [9] Costalos C(2003)Intestinal epithelial cells and innate immunity in the intestine: Is CARD15/Nod2 another player? Gastroenterology 124 1145-1149
  • [10] Israel EJ(2003)CARD15/NOD2 functions as an antibacterial factor in human intestinal epithelial cells Gastroenterology 124 993-1000
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