Crypt1, an active Ac-like transposon from the chestnut blight fungus, Cryphonectria parasitica

被引：13

作者：

Linder-Basso D. ^{[1
]}

Foglia R. ^{[1
]}

Zhu P. ^{[1
]}

Hillman B. ^{[1
]}

机构：

[1] Department of Plant Pathology, Cook College, Rutgers University, New Brunswick, NJ 08901-8520

来源：

Molecular Genetics and Genomics | 2001年 / 265卷 / 4期

关键词：

Chestnut blight; Cryphonectria parasitica; Filamentous fungi; Fungal virus; Population biology; Transposon;

D O I：

10.1007/s004380100470

中图分类号：

学科分类号：

摘要：

A moderately repetitive element was identified previously in the nuclear genome of the chestnut blight fungus, Cryphonectria parasitica, and has been used as a probe for population studies of the fungus. We report here that the repetitive element is a class II transposon of the hAT family of Activator (Ac)-like transposable elements. The element, named Crypt1, has a size of 3563 bp, including 21-bp terminal inverted repeats. A unique 8-bp direct repeat sequence flanking Crypt1 was identified in each of three clones examined. A single large ORF with the potential to encode a putative transposase of 946 amino acid residues was deduced from the sequence of Crypt1. Based on amino acid sequence alignments, Crypt1 is most closely related to other Ac-like transposons of filamentous ascomycetes. A single transcript of approximately 3.0 kb was identified by Northern hybridization experiments from Crypt1-containing isolates, suggesting that Crypt1 is an active element. An isolate containing a single, possibly defective, copy of Crypt1 was identified in C. parasitica isolates from China; no Crypt1 transcript was identified in this isolate. Transposition of Crypt1 was inferred from Southern and inverse PCR analyses of C. parasitica isolates maintained in the laboratory, but transposition appears to be a rare event.

引用

页码：730 / 738

页数：8

共 30 条

[11]

Hillman B.I., Fulbright D.W., Nuss D.L., Van Alfen N.K., Hypoviridae, Sixth Report of the International Committee for the Taxonomy of Viruses, (1995)

[12]

Hua-Van A., Daviere J.M., Kaper F., Langin T., Daboussi M.J., Genome organization in Fusarium oxysporum: Clusters of class II transposons, Curr Genet, 37, pp. 339-347, (2000)

[13]

Kempken F., Kuck U., restless, an active Ac-like transposon from the fungus Tolypocladium inflatum: Structure, expression, and alternative RNA splicing, Mol Cell Biol, 16, pp. 6563-6572, (1996)

[14]

Kempken F., Kuck U., Transposons in filamentous fungi - Facts and perspectives, BioEssays, 20, pp. 652-659, (1998)

[15]

Kempken F., Kuck U., Tagging of a nitrogen pathway-specific regulator gene in Tolypocladium inflatum by the transposon Restless, Mol Gen Genet, 263, pp. 302-308, (2000)

[16]

Kempken F., Jacobsen S., Kuck U., Distribution of the fungal transposon Restless: Full-length and truncated copies in closely related strains, Fungal Genet Biol, 25, pp. 110-118, (1998)

[17]

Kunze R., The maize transposable element Activator (Ac), pp. 161-194, (1996)

[18]

Milgroom M.G., Cortesi P., Analysis of population structure of the chestnut blight fungus based on vegetative incompatibility genotypes, Proc Natl Acad Sci USA, 96, pp. 10518-10523, (1999)

[19]

Milgroom M.G., Lipari S.E., Population differentiation in the chestnut blight fungus, Cryphonectria parasitica, in eastern North America, Phytopathology, 85, pp. 155-160, (1995)

[20]

Milgroom M.G., Lipari S.E., Powell W.A., DNA fingerprinting and analysis of population structures of the chestnut blight fungus, Cryphonectria parasitica, Genetics, 131, pp. 297-306, (1992)

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