Hepatic expression profile of forkhead transcription factor genes in normal Balb/c mice and their dynamic changes after bile duct ligation

被引：0
作者：
Ping Yang
Shifeng Huang
Ding Liu
Qianyun Zhou
Yang-an Wen
Yu Xiang
Shan Sun
Xiaofei Lai
Yufang Dong
Liping Zhang
机构：
[1] Chongqing Medical University,Department of Clinical Laboratory, the First Affiliated Hospital
[2] Third Military Medical University,Department of Laboratory Medicine, Affiliated Daping Hospital
来源：
Molecular Biology Reports | 2011年 / 38卷
关键词：
Bile duct ligation; Liver fibrosis; Forkhead transcription factor; Gene expression;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Dysregulation of Forkhead box (Fox) transcription factor family genes was previously shown to lead to congenital disorders, diabetes mellitus, and carcinogenesis, and recent reports suggested that several Fox genes play important roles in the pathogenesis of liver fibrosis. The present study was initiated to determine the expression profiles of the Fox genes in normal Balb/c mouse liver and their dynamic expression changes during fibrogenesis induced by experimental bile duct ligation (BDL). RT-PCR was employed to detect 18 FOX family members including FOXO1, FOXO3, FOXM1, and FOXL1 in normal mouse liver. FQ-PCR was performed to analyze the dynamic mRNA expression changes of nine inflammation- or proliferation-related FOX family genes in BDL mice. Results showed that all the 18 Fox genes were expressed in the normal mouse liver, among which the expression of FOXO1 and FOXO3 were found to be the highest. The inflammation and proliferation-related FOX family genes were found to be dynamically changed during BDL-induced liver injury with reduced FOXO1 and enhanced FOXOL1 and FOXM1, indicating their potential involvement in the pathogenesis of liver fibrosis. This is the first systematic evaluation of hepatic expression of FOX genes in both normal and BDL mice.
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页码：2665 / 2671
页数：6
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  • [1] Friedman SL(2004)Mechanisms of disease: mechanisms of hepatic fibrosis and therapeutic implications Nat Clin Pract Gastroenterol Hepatol 1 98-105
  • [2] Friedman SL(2000)Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury J Biol Chem 275 2247-1675
  • [3] Hsieh CS(2006)Glucocorticoid pretreatment suppresses chemokine expression and inflammatory cell infiltration in cholestatic rats receiving biliary intervention J Pediatr Surg 41 1669-1512
  • [4] Wang PW(2006)Hepatocyte growth factor attenuates liver fibrosis induced by bile duct ligation Am J Pathol 168 1500-1500
  • [5] Lee SY(2004)Human FOX gene family Int J Oncol 25 1495-22
  • [6] Huang CC(2010)The Fox genes in the liver: from organogenesis to functional integration Physiol Rev 90 1-1446
  • [7] Chang NK(2007)The forkhead transcription factor FoxO1 regulates proliferation and transdifferentiation of hepatic stellate cells Gastroenterology 132 1434-240
  • [8] Chen CM(2009)The evolution of Fox genes and their role in development and disease Nat Rev Genet 10 233-429
  • [9] Wu CL(2006)Loss of MMP13 attenuates murine hepatic injury and fibrosis during cholestasis Hepatology 44 420-747
  • [10] Wang HC(2009)Enhanced glucose synthesis in three-dimensional hepatocyte collagen matrix Toxicol In Vitro 23 744-929
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