Fluorescence fluctuation spectroscopy: Ushering in a new age of enlightenment for cellular dynamics

被引：40
作者：
Jameson D.M. [1 ]
Ross J.A. [1 ]
Albanesi J.P. [2 ]
机构：
[1] Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, 96813, 651 Ilalo St.
[2] Department of Pharmacology, U.T. Southwestern Medical Center, Dallas, TX, 75390-9041
来源：
Biophysical Reviews | 2009年 / 1卷 / 3期
基金：
美国国家卫生研究院;
关键词：
Fluorescence correlation spectroscopy; Fluorescence fluctuation spectroscopy; Number and brightness technique; Photon counting histogram; Raster scanning image correlation spectroscopy; Scanning FCS;
D O I：
10.1007/s12551-009-0013-8
中图分类号：
学科分类号：
摘要：
Originally developed for applications in physics and physical chemistry, fluorescence fluctuation spectroscopy is becoming widely used in cell biology. This review traces the development of the method and describes some of the more important applications. Specifically, the methods discussed include fluorescence correlation spectroscopy (FCS), scanning FCS, dual color cross-correlation FCS, the photon counting histogram and fluorescence intensity distribution analysis approaches, the raster scanning image correlation spectroscopy method, and the Number and Brightness technique. The physical principles underlying these approaches will be delineated, and each of the methods will be illustrated using examples from the literature. © 2009 International Union for Pure and Applied Biophysics (IUPAB) and Springer.
引用
收藏
页码：105 / 118
页数：13
相关论文
共 103 条
  • [1] Bacia K., Schwille P., A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy, Methods, 29, pp. 74-85, (2003)
  • [2] Bacia K., Majoul I.V., Schwille P., Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis, Biophys J, 83, pp. 1184-1193, (2002)
  • [3] Bacia K., Kim S.A., Schwille P., Fluorescence cross-correlation spectroscopy in living cells, Nat Methods, 3, pp. 83-89, (2006)
  • [4] Banks D.S., Fradin C., Anomalous diffusion of proteins due to molecular crowding, Biophys J, 89, pp. 2960-2971, (2005)
  • [5] Bark N., Foldes-Papp Z., Rigler R., The incipient stage in thrombin-induced fibrin polymerization detected by FCS at the single molecule level, Biochem Biophys Res Commun, 260, pp. 35-41, (1999)
  • [6] Berland K.M., Detection of specific DNA sequences using dual-color two-photon fluorescence correlation spectroscopy, J Biotechnol, 108, pp. 127-136, (2004)
  • [7] Berland K.M., So P.T., Gratton E., Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment, Biophys J, 68, pp. 694-701, (1995)
  • [8] Berland K.M., So P.T., Chen Y., Mantulin W.W., Gratton E., Scanning two-photon fluctuation correlation spectroscopy: particle counting measurements for detection of molecular aggregation, Biophys J, 71, pp. 410-420, (1996)
  • [9] Bestvater F., Spiess E., Stobrawa G., Hacker M., Feurer T., Porwol T., Berchner-Pfannschmidt U., Wotzlaw C., Acker H., Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging, J Microsc, 208, pp. 108-115, (2002)
  • [10] Borejdo J., Motion of myosin fragments during actin-activated ATPase: fluorescence correlation spectroscopy study, Biopolymers, 18, pp. 2807-2820, (1979)
12345678910