Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: a histological and histometric study in rat calvaria

被引：0
作者：
Valdir Gouveia Garcia
Juliana Mendonça da Conceição
Leandro Araújo Fernandes
Juliano Milanezi de Almeida
Maria José Hitomi Nagata
Alvaro Francisco Bosco
Leticia Helena Theodoro
机构：
[1] São Paulo State University (UNESP)—UNESP,GEPLO—Group of Study and Research on Laser in Dentistry, Department of Surgery and Integrated Clinic—Division of Periodontics
[2] Federal University of Alfenas,Department of Clinic and Surgery
[3] University Center of the Educational Foundation of Barretos (UNIFEB),Department of Periodontics
[4] Barretos Dental School,undefined
[5] Faculdade de Odontologia de Araçatuba—UNESP,undefined
来源：
Lasers in Medical Science | 2013年 / 28卷
关键词：
Alendronate; Bisphosphonate; Bone regeneration; Laser therapy; Rats;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The purpose of this study was to analyze histologically the effect of low-level laser therapy (LLLT) in combination with bisphosphonate on bone healing in surgically created critical size defects (CSD) in rat calvaria. One hundred Wistar female rats sham operated (sham) and ovariectomized (Ovx) were maintained untreated for 1 month to allow for the development of osteopenia in the Ovx animals. A CSD was made in the calvarium of each rat, and the animals were divided into five groups according to following treatments: (1) sham rats (control), (2) Ovx rats, (3) Ovx rats treated with LLLT, (4) Ovx rats treated with bisphosphonate, and (5) Ovx rats treated with bisphosphonate and LLLT. Groups 4 and 5 were irrigated with 1 ml of bisphosphonate, and groups 3 and 5 were submitted to LLLT (GaAlAs), 660 nm, 24 J, and 0.4285 W/cm2 on the CSD. Ten animals of each treatment were killed at 30 and 60 days. Histomorphometric assessments, using image analysis software, and histological analyses were performed. No defect was completely regenerated with the bone. Histometrically, it can be observed that groups 3 (37.49 ± 1.94%, 43.11 ± 2.39%) and 5 (35.05 ± 1.57%, 41.07 ± 1.89%) showed a significant bone neoformation when compared to groups 1 (16.81 ± 1.57%, 27.54 ± 1.49%), 2 (11.68 ± 0.98%, 22.51 ± 1.05%), and 4 (14.62 ± 1.70%, 25.67 ± 1.41%) in all experimental periods (P < 0.05). It was possible to conclude that the LLLT associated or not with bisphosphonate treatment was effective for stimulating bone formation in CSD in the calvaria of rats submitted to ovariectomy.
引用
收藏
页码：407 / 414
页数：7
相关论文
共 202 条
[1]  
Mangolas SC(2000)Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis Endocr Rev 21 115-137
[2]  
Gali JC(2001)Osteoporose Acta Ortop Bras 9 53-62
[3]  
Friedlander AH(1993)The physiology, medical management and oral implications of menopause Am J Med 95A 5S-16S
[4]  
Botell M(2002)Osteoporosis en la menopausia, prevención y estratégias terapêuticas atuales J Am Dent Assoc 133 73-81
[5]  
Park-Wyllie LY(2001)Bisphosphonate use and the risk of subtrochanteric or femoral shaft fractures in older women Rev Cub Obst Ginecol 27 199-204
[6]  
Mamdani MM(2011)From estrogen-centric to aging and oxidative stress: a revised perspective of the pathogenesis of osteoporosis JAMA 305 783-789
[7]  
Juurlink DN(2010)Effect of sodium alendronate on alveolar bone resorption in experimental periodontitis in rats Endocr Rev 31 266-300
[8]  
Hawker GA(2005)Alendronate prevents bone loss and improves tendon-to-bone repair strength in a canine model Periodontol 2000 76 1901-1909
[9]  
Gunraj N(2007)The antiresorptive effects of a single dose of zoledronate persist for two years: a randomized, placebo-controlled trial in osteopenic postmenopausal women J Orthop Res 25 473-479
[10]  
Austin PC(2009)Effect of low power laser irradiation on the mechanical properties of bone fracture healing in rats J Clin Endocrinol Metab 94 538-544
12345678910