Visualization of enzymatic DNA extension by surface plasmon resonance imaging

被引：0
作者：
Jaeyeon Jung
Jinho Hyun
机构：
[1] Seoul National University,Department of Biosystems and Biomaterials Science and Engineering
[2] Seoul National University,Research Institute for Agriculture and Life Sciences
来源：
BioChip Journal | 2011年 / 5卷
关键词：
Microbead; Enzymatic extension; DNA; Surface plasmon resonance (SPR); Imaging;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The enzymatic extension of single-stranded DNA on a gold substrate was studied with surface plasmon resonance (SPR) imaging and atomic force microscopy. Extension of the DNA strands was carried out by TdT, which catalyzes the addition of different deoxynucleotidyl triphosphates (dNTPs) to the 3′-hydroxyl end of DNA. The extension rate of the DNA strands depended on the dNTPs, in the order of dATP ≈dTTP>dCTP>dGTP. The time-dependence of the DNA extension was imaged using SPR.
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页码：304 / 308
页数：4
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