Investigation of Coxiella burnetii in Iranian camels

被引：21
作者：
Doosti A. [1 ]
Arshi A. [1 ]
Sadeghi M. [1 ]
机构：
[1] Biotechnology Research Center, Islamic Azad University, Shahrekord, Shahrekord Branch
来源：
Comparative Clinical Pathology | 2014年 / 23卷 / 1期
关键词：
Camel; Coxiella burnetii; Iran; PCR;
D O I：
10.1007/s00580-012-1567-6
中图分类号：
学科分类号：
摘要：
Coxiella burnetii is a zoonotic, obligate intracellular bacterium that caused Q fever. Antibodies to this organism have been reported in a wide range of animals including mammals, reptiles, amphibians, and birds. This study is aimed to detect C. burnetii in camel by polymerase chain reaction (PCR). Blood samples from 130 camels were collected between August and September 2011 then examined in laboratory conditions. Detection of the presence of C. burnetii DNA was carried out using a PCR assay with specific primers (Coc-F and Coc-R) targeting the 16S ribosomal RNA gene (242 bp). In this study, a total of 14 (10.76 %) camel blood samples were found PCR positive for C. burnetii. This result proves that camels are an important reservoir of C. burnetii infection. This study showed relatively high positivity of C. burnetii in Iranians camels, and accordingly, it seems necessary to evaluate the prevalence for this microorganism in Iran. © 2012 Springer-Verlag London Limited.
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页码：43 / 46
页数：3
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