Identification of an anther-specific promoter from a male sterile AB line in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

The promoter of the male sterile gene is important for studying male sterility. In this study, BraA08g014780.3C which differentially expressed between male sterile and fertile plants was identified from a genetic male sterile AB line of Chinese cabbage by RNA-seq. qRT-PCR revealed that BraA08g014780.3C was mainly expressed in the early stage of floral bud development in fertile plants, and preferentially expressed in their anthers. The promoter of BraA08g014780.3C was cloned and analyzed. Cis acting element analysis showed that the promoter of BraA08g014780.3C contains POLLEN1LELAT52 and GTGANTG10, which are both pollen-specific expression elements. The BraA08g014780.3Cp::GUS vector was constructed, then transformed to Arabidopsis thaliana Col-0. PCR analysis and sequencing of the transgenic Arabidopsis revealed that the GUS gene driven by the BraA08g014780.3C promoter was successfully transformed to the Arabidopsis. GUS staining indicated that the promoter of BraA08g014780.3C was an anther-specific promoter. Identifying the anther-specific promoter laid a foundation for revealing BraA08g014780.3C function in male sterility of Chinese cabbage.