The analysis of auxin distribution in the wild type and abruptus mutant plants of Arabidopsis thaliana (L.) heynh. By using the chimeric gene DR5::GUS

被引:1
作者
U. N. Kavai-ool
T. A. Ezhova
机构
[1] Tuva State University,Department of Genetics
[2] Moscow State University,undefined
关键词
plant development; auxin; mutants; transgenic plants;
D O I
10.3103/S0096392510030041
中图分类号
学科分类号
摘要
We have studied the activity of the chimeric gene DR5::GUS that indicates the spatial pattern of free auxin distribution and relative auxin level in the tissues of A. thaliana plants. It is established that the temperature sensitive abruptus mutation leads to temperature dependent increased accumulation of the free auxin in leaves, staments, and sepals in comparison to control plants homozygous for the wild type allele ABRUPTUS/PINOID. Our data revealed the important role of the ABRUPTUS/PINOID gene in the regulation of the auxin polar transport.
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页码:104 / 106
页数:2
相关论文
共 34 条
[1]  
Friml J.(2004)A Science 306 862-865
[2]  
Yang X.(1995) Dependent Binary Switch in Apical-Basal PIN Polar Targeting Directs Auxin Efflux Plant J. 8 505-520
[3]  
Michniewicz M.(2000)Morphogenesis in Pinoid Mutants of Cell 100 469-478
[4]  
Bennett S.R.M.(1997)Regulation of Auxin Response by the Protein Kinase Dokl. Akad. Nauk 354 839-842
[5]  
Alvarez J.(1997)Study of the Role of the Plant Cell 9 1963-1971
[6]  
Bossinger G.(1960) Gene in Differentiation of Flower-Bearing Stems in Vestn. Len. Univ., Ser. Biol. 15 47-56
[7]  
Smyth D.R.(2006) (L.) Heynh. Planta 223 315-328
[8]  
Christensen S.K.(2002)Aux/IAA Proteins Repress Expression of Reporter Genes Containing Natural and Highly Active Synthetic Auxin Response Elements Genes Dev. 16 3100-3112
[9]  
Dagenais N.(2000)Aseptic Culture of Vestn. SPbGU, Ser. 3 1 42-49
[10]  
Chory J.(2000) (L.) Heynh. and the Prospects of Its Use in Botanical Studies Genetika 36 1-6