Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation

被引：0
作者：
Alexandra V. Litvinchuk
J. Vachelová
A. Michaelidesová
R. Wagner
M. Davídková
机构：
[1] Nuclear Physics Institute CAS,Department of Radiation Dosimetry
[2] Proton Therapy Center,undefined
来源：
Radiation and Environmental Biophysics | 2015年 / 54卷
关键词：
Human fibroblasts; Proton radiation; Micronuclei assay; Biodosimetry;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Micronuclei are small extranuclear bodies resulting from chromosome fragments or the whole chromosomes secluded from daughter nuclei during mitosis. The number of radiation-induced micronuclei reflects the level of chromosomal damage and relates to an absorbed dose and quality of incident ionizing radiation. The aim of the present study was to determine the micronucleus formation as a specific biological marker for acute radiation-induced DNA damage in normal human fibroblasts exposed to 30-MeV protons and Co-60 gamma radiation. We found a linear increase in binuclear cells containing micronuclei for absorbed doses from 1 to 5 Gy for both radiation modalities. However, the total number of micronuclei in binuclear cells follows a linear-quadratic dose dependence. In case of human exposure to mixed radiation fields or high LET radiation, the proportion of binuclear cells containing micronuclei from all binuclear cells can thus serve as a good biomarker of radiation-induced DNA damage.
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页码：327 / 334
页数：7
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共 261 条
[1]  
Ainsbury EA(2011)Review of retrospective dosimetry techniques for external ionising radiation exposures Radiat Prot Dosim 147 573-592
[2]  
Bakhanova E(1980)Multinucleate cells and micronucleus formation in cultured human cells exposed to 12 MeV protons and gamma-rays Int J Radiat Biol Relat Stud Phys Chem Med 37 1-9
[3]  
Barquinero JF(1981)Chromosome aberrations induced by protons up to 31 MeV in cultured human cells Radiat Environ Biophys 19 91-100
[4]  
Brai M(2007)An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans Carcinogenesis 28 625-631
[5]  
Chumak V(1976)The production of micronuclei from chromosomal aberrations in irradiated cultures of human lymphocytes Mutat Res 41 321-332
[6]  
Correcher V(2009)Lethal events in V79 cells irradiated by low-energy protons and correlations with distribution patterns of energy deposition, radical concentration and DNA damage Appl Radiat Isot 67 454-459
[7]  
Darroudi F(2010)Current status of biodosimetry based on standard cytogenetic methods Radiat Environ Biophys 49 567-581
[8]  
Fattibene P(2007)Apoptosis and micronuclei induction in human epithelial cells exposed to energetic carbon ions in the Bragg peak region Adv Space Res 40 501-505
[9]  
Gruel G(1985)Measurement of micronuclei in lymphocytes Mutat Res 147 29-36
[10]  
Guclu I(1999)The Human Micronucleus Project—an international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans Mutat Res 428 271-283
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