Palmitate Enhances Protein Tyrosine Phosphatase 1B (PTP1B) Gene Expression at Transcriptional Level in C2C12 Skeletal Muscle Cells

被引：0
作者：
Narges MohammadTaghvaei
Reza Meshkani
Mohammad Taghikhani
Bagher Larijani
Khosrow Adeli
机构：
[1] Tarbiat Modares University,Department of Clinical Biochemistry, Faculty of Medical Sciences
[2] Tehran University of Medical Sciences,Department of Biochemistry, Faculty of Medicine
[3] Tehran University of Medical Sciences,Endocrinology and Metabolism Research Centre, Shariati Hospital
[4] University of Toronto,Clinical Biochemistry, The Hospital for Sick Children
来源：
Inflammation | 2011年 / 34卷
关键词：
protein tyrosine phosphatase 1B; insulin resistance; palmitate; macrophage; C2C12 myotube; inflammation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The factors responsible for regulating PTP1B expression in certain metabolic states have been unclear. We investigated the effects of palmitate on PTP1B gene transcription in C2C12 myotubes and myotubes co-cultured with J774A.1 macrophages. PTP1B mRNA and promoter activity levels were measured in C2C12 myotubes and direct or indirect co-culture of myotubes with macrophages using real time PCR and luciferase activity, respectively. Palmitate significantly induced PTP1B mRNA and promoter activity levels 1.36- and 1.32-fold, respectively, in C2C12 cells compared with the untreated myotubes. Palmitate showed a 2.3-fold increase in PTP1B mRNA level in myotubes co-cultured indirectly with macrophages. PTP1B promoter activity was also significantly induced by palmitate in direct (1.8-fold) and indirect (1.48-fold) co-culture of myotubes with macrophages. These data suggest that while palmitate treatment significantly induced PTP1B expression at the transcriptional level, the presence of an inflammatory process exerted by macrophages can augment this effect in the muscle cells.
引用
收藏
页码：43 / 48
页数：5
相关论文
共 175 条
[1]  
Kelley DE(2002)Muscle triglyceride and insulin resistance Annual Review of Nutrition 22 325-346
[2]  
Goodpaster BH(1985)Effects of insulin on peripheral and splanchnic glucose metabolism in noninsulin-dependent (type II) diabetes mellitus Journal of Clinical Investigation 76 149-155
[3]  
Storlien L(2008)Mechanisms of disease: molecular and metabolic mechanisms of insulin resistance and beta-cell failure in type 2 diabetes Nature Reviews. Molecular Cell Biology 9 193-205
[4]  
Defronzo R(2006)Pathophysiology of insulin resistance Best Pract Res Clin Endocrinol Metabol 20 665-679
[5]  
Gunnarsson R(2002)Coordinated action of protein tyrosine phosphatases in insulin signal transduction European Journal of Biochemistry 269 1050-1059
[6]  
Bjorkman O(2004)Transgenic overexpression of protein-tyrosine phosphatase 1B in muscle causes insulin resistance, but overexpression with leukocyte antigen-related phosphatase does not additively impair insulin action Journal of Biological Chemistry 279 24844-24851
[7]  
Olsson M(2005)Involvement of the small protein tyrosine phosphatases TC-PTP and PTP1B in signal transduction and diseases: from diabetes, obesity to cell cycle, and cancer Biochimica et Biophysica Acta 1754 108-117
[8]  
Wahren J(2005)Cytoplasmic protein tyrosine phosphatases, regulation and function: the roles of PTP1B and TC-PTP Current Opinion in Cell Biology 17 203-209
[9]  
Muoio D(1999)Increased insulin sensitivity and obesity resistance in mice lacking the protein tyrosine phosphatase-1B gene Science 283 1544-1548
[10]  
Newgard C(2000)Increased energy expenditure, decreased adiposity, and tissue-specific insulin sensitivity in protein-tyrosine phosphatase 1B-deficient mice Molecular and Cellular Biology 20 5479-5489
12345678910