Mechanical Loading Promotes the Migration of Endogenous Stem Cells and Chondrogenic Differentiation in a Mouse Model of Osteoarthritis

被引：0
作者：
Jie Li
Xiaoyu Wang
Xinle Li
Daquan Liu
Lidong Zhai
Xuetong Wang
Ran Kang
Hiroki Yokota
Lei Yang
Ping Zhang
机构：
[1] Tianjin Medical University,Department of Anatomy and Histology, School of Basic Medical Sciences
[2] Tianjin Medical University,Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases
[3] Tianjin Medical University,Research Center of Basic Medical Sciences
[4] Indiana University-Purdue University Indianapolis,Department of Biomedical Engineering
[5] Hebei University of Technology,Center for Health Sciences and Engineering, Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Health Sciences and Biomedical Engineering
[6] Tianjin Medical University,Tianjin Key Laboratory of Spine and Spinal Cord
来源：
Calcified Tissue International | 2023年 / 112卷
关键词：
Osteoarthritis; Endogenous stem cells; Piezo1; SDF-1; CXCR4; SOX9; Mechanical loading;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Osteoarthritis (OA) is a major health problem, characterized by progressive cartilage degeneration. Previous works have shown that mechanical loading can alleviate OA symptoms by suppressing catabolic activities. This study evaluated whether mechanical loading can enhance anabolic activities by facilitating the recruitment of stem cells for chondrogenesis. We evaluated cartilage degradation in a mouse model of OA through histology with H&E and safranin O staining. We also evaluated the migration and chondrogenic ability of stem cells using in vitro assays, including immunohistochemistry, immunofluorescence, and Western blot analysis. The result showed that the OA mice that received mechanical loading exhibited resilience to cartilage damage. Compared to the OA group, mechanical loading promoted the expression of Piezo1 and the migration of stem cells was promoted via the SDF-1/CXCR4 axis. Also, the chondrogenic differentiation was enhanced by the upregulation of SOX9, a transcription factor important for chondrogenesis. Collectively, the results revealed that mechanical loading facilitated cartilage repair by promoting the migration and chondrogenic differentiation of endogenous stem cells. This study provided new insights into the loading-driven engagement of endogenous stem cells and the enhancement of anabolic responses for the treatment of OA.
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页码：363 / 376
页数：13
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