Sequence and phylogenetic analysis of the cytoplasmic inclusion protein gene of zucchini yellow mosaic potyvirus: Its role in classification of the potyviridae

被引：8
作者：
Lee K.-C. [1 ]
Mahtani P.H. [1 ]
Chng C.-G. [1 ]
Wong S.-M. [1 ]
机构：
[1] School of Biological Sciences, National University of Singapore, Kent Ridge
来源：
Virus Genes | 1997年 / 14卷 / 1期
关键词：
CI gene; Classification; Nucleotide binding motif; Phylogenetic analysis; Potyviridae; Zucchini yellow mosaic virus;
D O I：
10.1023/A:1007935423180
中图分类号：
学科分类号：
摘要：
The cytoplasmic inclusion (CI) gene of a Singapore isolate of zucchini yellow mosaic virus (ZYMV-S) was sequenced and compared with CI of 14 other potyviruses. In addition to the consensus sequence GAVGSGKST of nucleotide binding motif (NTBM) which is implicated as a membrane-binding component of the RNA helicase complex, five other conserved motifs were found. Phylogenetic trees were constructed from sequence data for the CI and the coat protein. Similar branching patterns obtained from both CI and coat protein analyses suggests that phylogenetic relationship among potyviruses can be determined using the CI. We propose that phylogenetic analysis of CI gene may be used as an alternative approach for the study of evolution within the family Potyviridae.
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页码：41 / 53
页数：12
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