Embryogenic mango cultures selected for resistance to Colletotrichum gloeosporioides culture filtrate show variation in random amplified polymorphic DNA (RAPD) markers

被引：10

作者：

Jayasankar S. ^{[1
]}

Litz R.E. ^{[1
]}

Schnell R.J. ^{[2
]}

Hernandez A.-C. ^{[3
]}

机构：

[1] Tropical Research and Education Center, University of Florida, Homestead, FL 33031-3314

[2] USDA ARS, Subtropical Horticulture Research Station, Miami, FL 33158

[3] CINVESTAV, Unidad Irapuato, Irapuato, Guanajuato

来源：

In Vitro Cellular & Developmental Biology - Plant | 1998年 / 34卷 / 2期

关键词：

Anthracnose; DNA markers; In vitro selection; Mangifera indica; Somatic embryo;

D O I：

10.1007/BF02822774

中图分类号：

学科分类号：

摘要：

Genomic DNA isolated from embryogenic cultures of two mango cultivars, 'Hindi' and 'Carabao,' that had been selected for resistance to the culture filtrate of Colletotrichum gloeosporioides, was analyzed using Randomly Amplified Polymorphic DNA (RAPD). In vitro selection caused changes in RAPD markers in the selected embryogenic cultures with respect to the unchallenged control cultures and the stock plants. The differences involved both the absence and the presence of additional RAPD markers in the resistant lines, although the former was most commonly observed. The absence of differences between the unchallenged control of either cultivar and DNA from the leaves of parent trees confirmed that the changes were not due to prolonged maintenance in liquid cultures.

引用

页码：112 / 116

页数：4

共 33 条

[1]

Ballio A., Bottalico A., Buonocore V., Et al., Production and isolation of aspergillomarasmin B (lycomarasmic acid) from cultures of Colletotrichum gloeosporioides Penz. (Gloesporum olivarum Alm.), Phytopathol. Mediterr., 8, pp. 187-196, (1969)

[2]

Bosquet J.F., Vegh I., Pouteau-Thouvenot M., Et al., Isolemant de l'aspergillomarasmine A de cultures de Colletotrichum gloeosporioides Penz. agent pathogene des saules, Ann. Phytopathol., 3, pp. 407-408, (1971)

[3]

Collins G.G., Symons R.H., Polymorphisms in grapevine DNA detected by the RAPD-PCR technique, Plant Mol. Biol. Rep., 11, pp. 105-112, (1993)

[4]

Dan Y.H., Stephens C.T., DNA polymorphisms in somaclonal variants of Asparagus officinalis L. resistant to Fusarium oxysporum f. sp. asparagi, Plant Tiss. Cult. Biotech., 2, pp. 89-96, (1997)

[5]

Deng Z.N., Gentile A., Domina F., Et al., Recovery of citrus somatic hybrids tolerant to Phoma tracheiphila toxin, combining selection and identification by RAPD markers, pp. 177-183, (1995)

[6]

Doyle J.J., Doyle J.L., Isolation of plant DNA from fresh tissue, Focus, 12, pp. 13-15, (1990)

[7]

Gohbara M., Kosuge Y., Yamasaki S., Et al., Isolation, structures and biological activities of colletotrichins, phytotoxic substances from Colletotrichum nicotianae, Agric. Biol. Chem., 42, pp. 1037-1043, (1978)

[8]

Harding K., The methylation status of DNA derived from potato plants recovered from stow growth, Plant Cell Tissue Organ Cult, 37, pp. 31-38, (1994)

[9]

Harding K., Benson E.E., Roubelakis-Angelakis A., Methylated DNA changes associated with the initiation and maintenance of Vitis vinifera in vitro shoot and callus cultures: A possible mechanism for age related changes, Vitis, 35, pp. 79-85, (1996)

[10]

Hashmi G., Huettel R., Meyer R., Et al., RAPD analysis of somaclonal variants derived from embryo callus cultures of peach, Plant Cell Rep., 16, pp. 624-627, (1997)

← 1 2 3 4 →