Partial polymerase gene sequence, phylogeny and RT-PCR diagnostic assay for Datura yellow vein nucleorhabdovirus

被引：2

作者：

Dietzgen R.G. ^{[1
,2
]}

Tan E.R. ^{[2
]}

Yong A.H.S. ^{[2
]}

Feng C.-W.A. ^{[2
]}

机构：

[1] Centre for Plant Science, Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St. Lucia

[2] School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia

来源：

Australasian Plant Disease Notes | 2013年 / 8卷 / 1期

关键词：

Datura stramonium; Degenerate primers; Rhabdovirus; Thunbergia alata; Virus detection;

D O I：

10.1007/s13314-012-0087-1

中图分类号：

学科分类号：

摘要：

Partial (c. 0.25 and 1.1 kb) nucleotide sequences of the L polymerase gene of Datura yellow vein virus (DYVV) were obtained using two sets of degenerate oligonucleotide primers. This is the first sequence information for this virus. Phylogenetic analysis of the conserved L gene sequence with those of other plant rhabdoviruses showed that DYVV is most closely related to Sonchus yellow net virus and confirmed its taxonomic placement in the genus Nucleorhabdovirus. DYVV strains isolated from Datura stramonium and Thungergia alata, respectively, were 97.2 % identical in nucleotide sequence across a 251 nt region of the L gene. DYVV-specific primers were designed and a diagnostic RT-PCR assay developed and validated. © 2012 Australasian Plant Pathology Society Inc.

引用

页码：21 / 25

页数：4

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