A mediator-free amperometric hydrogen peroxide biosensor based on HRP immobilized on a nano-Au/poly 2,6-pyridinediamine-coated electrode

被引：0
作者：
Shurui Cao
Ruo Yuan
Yaqin Chai
Lingyan Zhang
Xuelian Li
Fengxian Gao
机构：
[1] Southwest University,Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering
来源：
Bioprocess and Biosystems Engineering | 2007年 / 30卷
关键词：
2,6-Pyridinediamine; Colloidal Au; Horseradish peroxidase; Biosensor; Electropolymerization;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A mediator-free amperometric hydrogen peroxide biosensor was prepared by immobilizing horseradish peroxidase (HRP) enzyme on colloidal Au modified platinum (Pt) wire electrode, which was modified by poly 2,6-pyridinediamine (pPA). The modified process was characterized by electrochemical impedance spectroscopy (EIS), and the electrochemical characteristics of the biosensor were studied by cyclic voltammetry, linear sweep voltammetry and chronoamperometry. The biosensor displayed an excellent electrocatalytical response to reduction of H2O2 without the aid of an electron mediator, the linear range was 4.2 × 10−7–1.5 × 10−3 mol/L (r = 0.9977), with a detection limit of 1.4 × 10−7 mol/L. Moreover, the performance and factors influencing the resulted biosensor were studied in detail. The studied biosensor exhibited permselectivity, good stability and good fabrication reproducibility.
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页码：71 / 78
页数：7
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