Live-cell super-resolution imaging with trimethoprim conjugates

被引：0
|
作者：
Wombacher R. [1 ,5 ]
Heidbreder M. [2 ]
Van De Linde S. [3 ]
Sheetz M.P. [4 ]
Heilemann M. [2 ]
Cornish V.W. [1 ]
Sauer M. [3 ]
机构：
[1] Department of Chemistry, Columbia University, New York, NY
[2] Applied Laser Physics and Laser Spectroscopy, Bielefeld University, Bielefeld
[3] Department of Biotechnology and Biophysics, Julius Maximilians University Wuerzburg, Wuerzburg
[4] Department of Biological Sciences, Columbia University, New York, NY
[5] Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg
来源：
Nature Methods | 2010年 / 7卷 / 9期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1038/nmeth.1489
中图分类号：
学科分类号：
摘要：
The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at ∼20 nm resolution. © 2010 Nature America, Inc. All rights reserved.
引用
收藏
页码：717 / 719
页数：2
相关论文
共 50 条
  • [1] Live-cell super-resolution imaging with trimethoprim conjugates
    Wombacher, Richard
    Heidbreder, Meike
    van de Linde, Sebastian
    Sheetz, Michael P.
    Heilemann, Mike
    Cornish, Virginia W.
    Sauer, Markus
    NATURE METHODS, 2010, 7 (09) : 717 - 719
  • [2] Super-resolution microscopy in live-cell imaging
    Hedde, Per Niklas
    Gayda, Susan
    Nienhaus, Karin
    Nienhaus, G. Ulrich
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2011, 40 : 151 - 152
  • [3] Fluorescent proteins for live-cell imaging with super-resolution
    Nienhaus, Karin
    Nienhaus, G. Ulrich
    CHEMICAL SOCIETY REVIEWS, 2014, 43 (04) : 1088 - 1106
  • [4] Live-Cell Super-Resolution Imaging with Synthetic Fluorophores
    van de Linde, Sebastian
    Heilemann, Mike
    Sauer, Markus
    ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 63, 2012, 63 : 519 - 540
  • [5] Live-Cell Super-Resolution Imaging Goes Multicolor
    Klein, Teresa
    van de Linde, Sebastian
    Sauer, Markus
    CHEMBIOCHEM, 2012, 13 (13) : 1861 - 1863
  • [6] Organic fluorescent probes for live-cell super-resolution imaging
    Xinxin Duan
    Meng Zhang
    Yu-Hui Zhang
    Frontiers of Optoelectronics, 16
  • [7] Organic fluorescent probes for live-cell super-resolution imaging
    Duan, Xinxin
    Zhang, Meng
    Zhang, Yu-Hui
    FRONTIERS OF OPTOELECTRONICS, 2023, 16 (01)
  • [8] Organic fluorescent probes for live-cell super-resolution imaging
    Xinxin Duan
    Meng Zhang
    Yu-Hui Zhang
    Frontiers of Optoelectronics, 2023, 16 (04) : 30 - 41
  • [9] Direct Live-Cell Super-Resolution Imaging of Cellular DNA
    Benke, Alexander
    Meylan, Xavier
    Manley, Suliana
    BIOPHYSICAL JOURNAL, 2012, 102 (03) : 223A - 223A
  • [10] Live-Cell Super-resolution Fluorescence Microscopy
    Mishin, A. S.
    Lukyanov, K. A.
    BIOCHEMISTRY-MOSCOW, 2019, 84 (Suppl 1) : 19 - 31
12345