Two-Photon and Second Harmonic Microscopy in Clinical and Translational Cancer Research

被引：0
作者：
Seth W. Perry
Ryan M. Burke
Edward B. Brown
机构：
[1] University of Rochester,Department of Biomedical Engineering
[2] University of Rochester School of Medicine and Dentistry,Department of Neurology
来源：
Annals of Biomedical Engineering | 2012年 / 40卷
关键词：
Two-photon microscopy; Cancer; Second harmonic generation; Collagen; SHG; Endoscopy;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Application of two-photon microscopy (TPM) to translational and clinical cancer research has burgeoned over the last several years, as several avenues of pre-clinical research have come to fruition. In this review, we focus on two forms of TPM—two-photon excitation fluorescence microscopy, and second harmonic generation microscopy—as they have been used for investigating cancer pathology in ex vivo and in vivo human tissue. We begin with discussion of two-photon theory and instrumentation particularly as applicable to cancer research, followed by an overview of some of the relevant cancer research literature in areas that include two-photon imaging of human tissue biopsies, human skin in vivo, and the rapidly developing technology of two-photon microendoscopy. We believe these and other evolving two-photon methodologies will continue to help translate cancer research from the bench to the bedside, and ultimately bring minimally invasive methods for cancer diagnosis and treatment to therapeutic reality.
引用
收藏
页码：277 / 291
页数：14
相关论文
共 582 条
  • [21] Pick R(2003)3-dimensional imaging of collagen using second harmonic generation J. Struct. Biol. 141 53-1330
  • [22] Hell SW(2009)Combined non-linear laser imaging (two-photon excitation fluorescence microscopy, fluorescence lifetime imaging microscopy, multispectral multiphoton microscopy) in cutaneous tumours: first experiences J. Eur. Acad. Dermatol. Venereol. (JEADV) 23 314-76
  • [23] Breunig HG(2010)Feasibility of using multiphoton excited tissue autofluorescence for in vivo human histopathology. Biomed Opt. Express 1 1320-515
  • [24] Studier H(1990)Two-photon laser scanning fluorescence microscopy Science 248 73-1758
  • [25] Konig K(2009)Spectral fluorescence lifetime detection and selective melanin imaging by multiphoton laser tomography for melanoma diagnosis Exp. Dermatol. 18 509-529
  • [26] Brown E(2009)Sensitivity and specificity of multiphoton laser tomography for in vivo and ex vivo diagnosis of malignant melanoma J. Invest. Dermatol. 129 1752-330
  • [27] McKee T(2010)Optical diagnostics of tissue pathology by multiphoton microscopy Expert Opin. Med. Diag. 4 519-2353
  • [28] diTomaso E(2008)Two-photon laser-scanning fluorescence microscopy applied for studies of human skin J. Biophotonics 1 320-950
  • [29] Pluen A(1971)Optical second harmonic generation in biological systems Appl. Opt. 10 2350-606
  • [30] Seed B(2008)Risk estimation of skin damage due to ultrashort pulsed, focused near-infrared laser irradiation at 800 nm J. Biomed. Opt. 13 041320-2988
12345678910