Hydrophilic interaction chromatography as an alternative to reversed-phase HPLC in determining anthocyanins and betacyanins

被引：0
作者：
V. I. Deineka
I. I. Saenko
L. A. Deineka
I. P. Blinova
机构：
[1] Belgorod State National Research University,Faculty of Biology and Chemistry
来源：
Journal of Analytical Chemistry | 2016年 / 71卷
关键词：
hydrophilic interaction chromatography; reversed-phase HPLC; anthocyanins; betacyanins; dependence of retention on structure;
D O I：
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学科分类号：
摘要：
We selected the conditions of hydrophilic interaction chromatography for separating anthocyanins and betacyanins using a diol stationary phase and mobile phases of acetonitrile–0.5 vol % phosphoric acid–water. Regularities of the separation of betacyanins and cyanidine derivatives of various structures are studied by analyzing their relative retentions. It is shown that the approach is efficient for the preliminary classification of anthocyanins by the type of glycosylation.
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页码：297 / 301
页数：4
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