Antigenic and genetic analysis of H3N8 influenza viruses isolated from horses in Japan and Mongolia, and imported from Canada and Belgium during 2007-2010

被引：0
作者：
Masayuki Motoshima
Masatoshi Okamatsu
Shingo Asakura
Saya Kuribayashi
Sugar Sengee
Damdinjav Batchuluun
Mika Ito
Yukiko Maeda
Mariko Eto
Yoshihiro Sakoda
Ruuragchaa Sodnomdarjaa
Hiroshi Kida
机构：
[1] Hokkaido University,Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine
[2] State Central Veterinary Laboratory,Animal Quarantine Service, Ministry of Agriculture
[3] Ishikawa Nanbu Livestock Hygiene Service Center,Research Center for Zoonosis Control
[4] Ishikari Livestock Hygiene Service Center,undefined
[5] Forestry and Fisheries,undefined
[6] Hokkaido University,undefined
来源：
Archives of Virology | 2011年 / 156卷
关键词：
Influenza; Influenza Virus; MDCK Cell; Antigenic Site; Neutralize Activity;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A/equine/Kanazawa/1/2007 (H3N8), A/equine/Hokkaido/I828/2008 (H3N8) and A/equine/Mongolia/1/2008 (H3N8) were isolated from infected horses. A/equine/Yokohama/aq19/2009 (H3N8) and A/equine/Yokohama/aq13/2010 (H3N8) were isolated from horses imported from Canada and Belgium examined at the Animal Quarantine Service in Yokohama, Japan. In the present study, these five isolates were genetically and antigenically analyzed. Phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed that three isolates from horses in Japan and imported from Canada belonged to the same branch, clade 1 of the Florida sublineage, while the isolates from horses in Mongolia and imported from Belgium belonged to another branch, clade 2 of the Florida sublineage. Reactivity patterns of a panel of monoclonal antibodies to the HA of A/equine/Kanazawa/1/2007 (H3N8) with the five isolates indicate that the HAs of these viruses were antigenically similar to each other and to the reference strains A/equine/La Plata/1/1993 (H3N8) and A/equine/Avesta/1/1993 (H3N8). The present findings indicate that extensive antigenic variation has not accumulated among H3N8 influenza viruses in horses.
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页码：1379 / 1385
页数：6
相关论文
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