Comparison between microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA) for detection of leptospiral antibodies in cattle

被引：5
作者：
Sakhaee E. [1 ]
Abdollahpour G. [2 ]
Bolourchi M. [2 ]
Tabrizi S.S. [2 ]
机构：
[1] Section of Large Animal Internal Medicine, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman
[2] Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran
来源：
Comparative Clinical Pathology | 2010年 / 19卷 / 1期
关键词：
Bovine; ELISA; Leptospirosis; MAT;
D O I：
10.1007/s00580-009-0907-7
中图分类号：
学科分类号：
摘要：
Leptospirosis is a worldwide zoonosis caused by Leptospira interrogans. It causes financial loss to the cattle industry with decreased milk production, abortion, stillbirth, infertility, and mortality. This study was conducted to evaluate two serological techniques, the microscopic agglutination test (MAT) and the enzyme-linked immunosorbent assay (ELISA), for detection of leptospiral antibodies in cattle. Three hundred and fifty five serum samples were collected from five commercial dairy farms in Tehran suburb from December 2004 to June 2005. The results of the MAT protocol showed that 55 serum samples (15.49%) had detectable antibodies against at least one serovar of L. interrogans at a dilution of ≥ 1:100. Icterohaemorrhagiae was the most (42.52%) and ballum the least (1.15%) prevalent serovars among 87 positive reactions against different serovars of L. interrogans. Leptospiral antibodies were also detected by the ELISA in 77 sera (21.69%) of 355 samples. The results of this study also showed that 2.25% of the sera that reacted positive in the MAT were negative by the ELISA, and 8.45% of the MAT negative sera were positive by the ELISA. Comparison between two techniques showed that there was a high correlation (0.61 < kappa = 0.649 < 0.8) between the ELISA and MAT for detection of leptospiral antibodies in cattle. © Springer-Verlag London Limited 2009.
引用
收藏
页码：5 / 9
页数：4
相关论文
共 22 条
  • [1] Abdollahpour G., Bovine Leptospirosis, with a Special Reference to Leptospira Interrogans Serovar Hardjo, (1995)
  • [2] Abdollahpour G., Shafighi S.T., Sattari S., Serodiagnosis of Leptospirosis in cattle in North of Iran, Guilan, International Journal of Veterinary Research, 3, 1, pp. 7-10, (2008)
  • [3] Adler B., Murphy A.M., Locarnini S.A., Faine S., Detection of specific anti leptospiral immunoglobulins M and G in human serum by solid-phase enzyme-linked immunosorbent assay, J Clin Microbiol, 11, pp. 452-457, (1980)
  • [4] Adler B., Faine S., Gordon L.M., The enzyme-linked immunosorbent assay (ELISA) as a serological test for detecting antibodies against Leptospira interrogans serovar hardjo in sheep, Aust Vet J, 57, pp. 414-417, (1981)
  • [5] Angela P., Brandao D., Camargo V., Marcos V., Macroscopic agglutination test for rapid diagnosis of human leptospirosis, J Clin Microbiol, 36, 11, pp. 3138-3142, (1998)
  • [6] Asadpour Y., Serological survey of leptospirosis in cattle in Shaft and Fuman in Gilan province, Proceedings of the 3rd Congress of Veterinary Clinicians in Iran, pp. 27-29, (1996)
  • [7] Cousins D.V., Robertson G.M., Hustas L., The use of the enzyme-linked immunosorbent assay (ELISA) to detect the IgM and IgG antibody response to Leptospira interrogans serovars hardjo, pomona and tarassovi in cattle, Vet Microbiol, 10, pp. 439-450, (1985)
  • [8] Durham P.J.K., Paine G.D., Serological survey for antibodies to infectious agents in beef cattle in Northern South Australia, Aust Vet J, 75, 2, pp. 134-140, (1997)
  • [9] Ebrahimi A., Alijani L., Abdollahpour G., Serological survey of human leptospirosis in tribal areas of Farsan and Koohrang cities, Iran J Med Sci, 28, 2, pp. 93-96, (2003)
  • [10] Ellis W.A., O'brien J.J., Neill S.D., Ferguson H.W., Hanna J., Bovine leptospirosis: Microbiological and serological findings in aborted fetuses, Vet Rec, 110, pp. 147-150, (1982)
123