Identification of RAPD and ISSR markers for drought tolerance in wheat (Triticum aestivum L.)

被引:8
作者
Reena Deshmukh
Nawab Singh Tomar
Niraj Tripathi
Sharad Tiwari
机构
[1] Biotechnology Centre, Jawaharlal Nehru Agricultural University
[2] Collage of Agriculture, Rajmata VRS Agricultural University
关键词
Drought; ISSR; Polygenic; RAPD;
D O I
10.1007/s12298-011-0096-0
中图分类号
学科分类号
摘要
Drought tolerance is the essential trait that needs to be incorporated in cereal crops, particularly those grown under the rainfed cultivation. Drought tolerance being contributed by several regions of the genome requires identification of these regions, using suitable molecular markers. Therefore, present investigation was aimed at analyzing the genetic diversity present among the cultivars of rainfed and the irrigated areas with respect to the drought tolerant trait. In all, 14 RAPD and 90 ISSR markers were used to identify these genomic regions. Out of 14 RAPD markers, one RAPD primer exhibited polymorphic banding pattern with 18. 6 % polymorphism, clearly separating drought tolerant and drought susceptible genotypes. Out of 90 ISSR primers, only 3 ISSR primers revealed polymorphism in relation to the drought tolerance trait exhibiting 21. 38 % polymorphism. © 2011 Prof. H.S. Srivastava Foundation for Science and Society.
引用
收藏
页码:101 / 104
页数:3
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