Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. cell suspension culture under magnetite nanoparticles and static magnetic field elicitation

被引：0
作者：
Marzieh Taghizadeh
Fatemeh Nasibi
Khosrow Manouchehri Kalantari
Faezeh Ghanati
机构：
[1] Shahid Bahonar University of Kerman,Department of Plant Biology
[2] Shahid Bahonar University of Kerman,Research and Technology Institute of Plant Production (RTIPP)
[3] Tarbiat Modares University,Department of Plant Biology, Faculty of Biological Science
来源：
Plant Cell, Tissue and Organ Culture (PCTOC) | 2019年 / 136卷
关键词：
Suspension cultured cells; Magnetite nanoparticles; Medicinal compounds; Static magnetic field; Antioxidant activity; Secondary metabolite;
D O I：
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中图分类号：
学科分类号：
摘要：
Suspension-cultured Dracocephlum polychaetum Bornm. is a wild medicinal herb native to Iran, treated with a static magnetic field (SMF) and Fe2O3 magnetic nanoparticles (MNP). The effect of SMF (30 mT), Fe2O3 MNP (100 ppm) and the combination of these treatments on phenolic metabolism and the medicinal compounds were examined by high performance liquid chromatography and UV–Vis spectrophotometer. The activity of polyphenol oxidase and phenylalanine ammonialyase, as well as the content of total phenolics, flavonoid, anthocyanins, lignin and malondialdehyde in all treated cells, showed a significant difference with control. The intracellular content of rosmarinic acid, naringin, apigenin, thymol, carvacrol, quercetin and rutin, in all treated cells increased considerably. Their quantities also increased significantly in the treated cell culture media. These results suggest this increase is due to the increased production and secretion of the medicinal compounds from cells to the culture media upon application of SMF and Fe2O3 MNP.
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页码：489 / 498
页数：9
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