Validation of a PCR-RFLP based method for the identification of salmon species in food products

被引：0

作者：

G. L. Hold

Valerie J. Russell

Susan E. Pryde

Hartmut Rehbein

Javier Quinteiro

Manuel Rey-Mendez

Carmen G. Sotelo

Ricardo I. Pérez-Martin

Ana T. Santos

Carla Rosa

机构：

[1] Rowett Research Institute,

[2] Greenburn Road,undefined

[3] Aberdeen,undefined

[4] AB21 9SB,undefined

[5] Scotland,undefined

[6] UK e-mail: glh@rri.sari.ac.uk,undefined

[7] Bundesforschungsanstalt für Fischerei,undefined

[8] Institut für Biochemie und Technologie,undefined

[9] Palmaille 9,undefined

[10] 22769 Hamburg,undefined

[11] Germany,undefined

[12] Departamento de Bioquimica y Biologia Molecular,undefined

[13] Facultad de Biologia,undefined

[14] Universidad de Santiago de Compostela,undefined

[15] 15706 Santiago de Compostela,undefined

[16] Spain,undefined

[17] Instituto de Investigaciones Marinas (CSIC),undefined

[18] Eduardo Cabello 6,undefined

[19] 36208 Vigo,undefined

[20] Spain,undefined

[21] Instituto de Investigação das Pescas e do Mar,undefined

[22] Av. Brasilia,undefined

[23] 1400 Lisbon,undefined

[24] Portugal,undefined

来源：

European Food Research and Technology | 2001年 / 212卷

关键词：

Keywords Salmon; Species identification; PCR-RFLP; Inter-laboratory study;

D O I：

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学科分类号：

摘要：

The use of a PCR-RFLP based method for the identification of salmon species in food products was investigated. The reliability and practicality of the method was tested by a collaborative study in which five European laboratories participated. Two unknown samples (a commercial product of known species composition and a mix of two salmon species) required identification by comparison with authentic reference species. From a total of 50 cases, 100% of authentic species were correctly assigned, with all unknown samples also correctly identified. A larger scale analysis of UK commercial products was also performed spanning the whole range of salmon products available. In almost all cases the salmon species declared was confirmed, although, a trout species was detected in one product declaring only the presence of salmon. The investigation confirms the reproducibility of the method in different laboratories, and its applicability for commercial product analysis.

引用

页码：385 / 389

页数：4