Analysis of the MCTP Amino Acid Sequence Reveals the Conservation of Putative Calcium- and Lipid-Binding Pockets Within the C2 Domains In Silico

被引:0
作者
José Luis Téllez-Arreola
Ataúlfo Martínez-Torres
Adriana E. Flores-Moran
José M. Lazaro-Guevara
Argel Estrada-Mondragón
机构
[1] Universidad Nacional Autónoma de México,Departamento de Neurobiología Celular Y Molecular, Instituto de Neurobiología
[2] Autonomous University of Queretaro,Unit for Basic and Applied Microbiology, School of Natural Sciences
[3] University of Utah,Department of Human Genetics
[4] University of British Columbia,Department of Botany
[5] University of British Columbia,Biodiversity Research Centre
[6] Linköping University,Department of Biomedical and Clinical Sciences (BKV)
来源
Journal of Molecular Evolution | 2022年 / 90卷
关键词
C2 domain; Calcium signaling; Docking; Membrane traffic;
D O I
暂无
中图分类号
学科分类号
摘要
MCTPs (Multiple C2 Domains and Transmembrane region Proteins) are evolutionarily and structurally related to other C2 proteins, which are central to exocytosis and membrane trafficking; however, their specific function has been little studied. MCTPs are associated with endosomes and the endoplasmic reticulum and possess three C2 domains (C2A-C2C) and two transmembrane regions (TMRs) well conserved in different species. Here, we generated structural models of the MCTP C2 domains of C. elegans and analyzed their putative function by docking, which revealed that these domains possess Ca2+- and lipid-binding pockets, suggesting that MCTPs play a significant, calcium-dependent role in membrane physiology.
引用
收藏
页码:271 / 282
页数:11
相关论文
共 419 条
  • [1] Alwarawrah M(2017)Investigation of the effect of bilayer composition on PKCα-C2 domain docking using molecular dynamics simulations J Phys Chem B 121 78-88
  • [2] Wereszczynski J(2009)Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains J Cell Biol 187 295-310
  • [3] Qiu X(2017)Intracellular Ca2+ sensing: its role in calcium homeostasis and signaling Mol Cell 66 780-788
  • [4] Ge J(2019)C2A activates a cryptic Ca(2+)-triggered membrane penetration activity within the C2B domain of synaptotagmin I Proc Natl Acad Sci U S A 93 1665-1670
  • [5] Gao Y(2001)Functional interaction of the active zone proteins Munc13-1 and RIM1 in synaptic vesicle priming Neuron 30 183-196
  • [6] Teng M(2014)Model species in evo-devo: a philosophical perspective Evol Dev 16 49-56
  • [7] Niu L(2019)Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata EMBO Rep 20 1-26
  • [8] Barber CF(2014)Signaling through C2 domains: more than one lipid target Biochim Biophys Acta Biomembr 1838 1536-1547
  • [9] Jorquera RA(2004)Structural basis for the evolutionary inactivation of Ca2+ binding to synaptotagmin 4 Nat Struct Mol Biol 11 844-849
  • [10] Melom JE(2018)RIM C 2 B domains target presynaptic active zone functions to PIP 2 -containing membranes Neuron 98 335-349.e7