Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis

被引：314
作者：
Atwood B.K. [1 ,3 ]
Lopez J. [2 ]
Wager-Miller J. [1 ]
Mackie K. [1 ]
Straiker A. [1 ]
机构：
[1] Department of Psychological and Brain Sciences, The Gill Center for Biomolecular Science, Indiana University, Bloomington, IN
[2] Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN
[3] Graduate Program in Neurobiology and Behavior, University of Washington, Seattle, WA
来源：
BMC Genomics | / 12卷 / 1期
基金：
美国国家卫生研究院;
关键词：
HEK293 Cell; AtT20 Cell; GPCR Signaling; Frizzle Receptor; Related Signaling Protein;
D O I：
10.1186/1471-2164-12-14
中图分类号：
学科分类号：
摘要：
Background: G protein coupled receptors (GPCRs) are one of the most widely studied gene superfamilies. Thousands of GPCR research studies have utilized heterologous expression systems such as human embryonic kidney cells (HEK293). Though often treated as 'blank slates', these cell lines nevertheless endogenously express GPCRs and related signaling proteins. The outcome of a given GPCR study can be profoundly influenced by this largely unknown complement of receptors and/or signaling proteins. Little easily accessible information exists that describes the expression profiles of the GPCRs in cell lines. What is accessible is often limited in scope - of the hundreds of GPCRs and related proteins, one is unlikely to find information on expression of more than a dozen proteins in a given cell line. Microarray technology has allowed rapid analysis of mRNA levels of thousands of candidate genes, but though often publicly available, the results can be difficult to efficiently access or even to interpret.Results: To bridge this gap, we have used microarrays to measure the mRNA levels of a comprehensive profile of non-chemosensory GPCRs and over a hundred GPCR signaling related gene products in four cell lines frequently used for GPCR research: HEK293, AtT20, BV2, and N18.Conclusions: This study provides researchers an easily accessible mRNA profile of the endogenous signaling repertoire that these four cell lines possess. This will assist in choosing the most appropriate cell line for studying GPCRs and related signaling proteins. It also provides a better understanding of the potential interactions between GPCRs and those signaling proteins. © 2011 Atwood et al; licensee BioMed Central Ltd.
引用
收藏
相关论文
共 76 条
[71]  
Hanham C.A., Zhao F., Tignor G.H., Evidence from the anti-idiotypic network that the acetylcholine receptor is a rabies virus receptor, J Virol, 67, 1, pp. 530-542, (1993)
[72]  
Howlett A.C., Fleming R.M., Cannabinoid inhibition of adenylate cyclase. Pharmacology of the response in neuroblastoma cell membranes, Mol Pharmacol, 26, 3, pp. 532-538, (1984)
[73]  
Roth B.L., Beinfeld M.C., Howlett A.C., Secretin receptors on neuroblastoma cell membranes: characterization of 125I-labeled secretin binding and association with adenylate cyclase, J Neurochem, 42, 4, pp. 1145-1152, (1984)
[74]  
Rice W.R., Burton F.M., Fiedeldey D.T., Cloning and expression of the alveolar type II cell P2u-purinergic receptor, Am J Respir Cell Mol Biol, 12, 1, pp. 27-32, (1995)
[75]  
Amano T., Richelson E., Nirenberg M., Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase-acetylcholinesterase-tyrosine hydroxylase-axons-dendrites), Proc Natl Acad Sci USA, 69, 1, pp. 258-263, (1972)
[76]  
Bono H., Yagi K., Kasukawa T., Nikaido I., Tominaga N., Miki R., Mizuno Y., Tomaru Y., Goto H., Nitanda H., Et al., Systematic expression profiling of the mouse transcriptome using RIKEN cDNA microarrays, Genome Res, 13, 6 B, pp. 1318-1323, (2003)
12345678