Optimization of amylase production by Aspergillus niger in solid-state fermentation using sugarcane bagasse as solid support material

被引：0
作者：
Renato Pérez Rosés
Nelson Pérez Guerra
机构：
[1] University of Oriente,Department of Pharmacy, Natural Science Faculty
[2] University of Vigo,Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Food Science and Technology Faculty, Ourense Campus
来源：
World Journal of Microbiology and Biotechnology | 2009年 / 25卷
关键词：
Amylase; Optimization; Solid-state fermentation; Sugarcane bagasse;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Synthesis of amylase by Aspergillus niger strain UO-01 under solid-state fermentation with sugarcane bagasse was optimized by using response surface methodology and empirical modelling. The process parameters tested were particle size of sugarcane bagasse, incubation temperature and pH, moisture level of solid support material and the concentrations of inoculum, total sugars, nitrogen and phosphorous. The optimum conditions for high amylase production (457.82 EU/g of dry support) were particle size of bagasse in the range of 6–8 mm, incubation temperature and pH: 30.2°C and 6.0, moisture content of bagasse: 75.3%, inoculum concentration: 1 × 107 spores/g of dry support and concentrations of starch, yeast extract and KH2PO4: 70.5, 11.59 and 9.83 mg/g of dry support, respectively. After optimization, enzyme production was assayed at the optimized conditions. The results obtained corroborate the effectiveness and reliability of the empirical models obtained.
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页码：1929 / 1939
页数：10
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共 110 条
[1]  
Abu EA(2004)Comparative studies on the effect of organic and inorganic nitrogen supplementation of millet and sorghum pomace on the production of three industrial enzymes by Biokemistry 16 64-70
[2]  
Ado SA(1991) SL.1 World J Microbiol Biotechnol 7 62-66
[3]  
Ali S(2007)Factors affecting cellulose production by Food Technol Biotechnol 45 439-442
[4]  
Sayed A(2003) using water hyacinth Process Biochem 38 1665-1668
[5]  
Sarker RT(2008)Production of α-amylase from Chem Biochem Eng Q 22 49-70
[6]  
Alam R(1994)Solid state fermentation for production of α-amylase by a thermotolerant Biotechnol Bioeng 44 240-247
[7]  
Balkan B(2002) from hot-spring water Process Biochem 38 615-620
[8]  
Ertan F(2002)Solid-state fermentation: an overview J Basic Microbiol 5 320-326
[9]  
Baysal Z(2003)Saccharification and fermentation of sugarcane bagasse Biochem Eng J 15 107-115
[10]  
Uyar F(2002)Optimization of process parameters for glucoamylase production under solid state fermentation by a new isolated Curr Microbiol 45 203-208
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