Presence and induction by bacteria of D-galactoside-specific lectins in the humoral fluids of amphioxus Branchiostoma belcheri tsingtauense

被引:3
作者
Wang C. [1 ]
Zhang S. [1 ]
Luo Y. [1 ]
Xu T. [1 ]
机构
[1] Department of Marine Biology, Ocean University of Qingdao
关键词
Amphioxus; Branchiostoma; Humoral fluid; Induction; Lancelet; Lectin;
D O I
10.1163/156856001760209789
中图分类号
学科分类号
摘要
The presence of lectins in the humoral fluids of amphioxus, an animal transitional from invertebrates to vertebrates, has thus far not been reported. It was shown here that lectins are present in the humoral fluids of amphioxus. The lectin activity in the humoral fluids of amphioxus is SH group-dependent and is specifically inhibited by various D-galactosides. Also, the humoral fluids obtained from E. coli-injected amphioxus showed increased agglutinating activity against human B and O, rabbit, grass carp and toad erythrocytes, suggesting that a form of adaptive response might exist in amphioxus.
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页码:241 / 248
页数:7
相关论文
共 25 条
[1]  
Barondes S.H., Bifunctional properties of lectins: Lectins redefined, Trends Biochem. Sci., 13, pp. 480-482, (1988)
[2]  
Barondes S.H., Cooper D.N., Gitt M.A., Et al., Galectins. Structure and function of a large family of animal lectins, J. Biol. Chem., 269, pp. 20807-20810, (1994)
[3]  
Benedictis G.D., Capalbo R., An amphioxus lanceolatus agglutinating factor for human red cells, J. Immunogenetics, 8, pp. 225-230, (1981)
[4]  
Drickamer K., Two distinct classes of carbohydrate-recognition domains in animal lectins, J. Biol. Chem., 263, pp. 9557-9560, (1988)
[5]  
Drickamer K., Taylor M.E., Biology of animal lectins, Annu. Rev. Cell Biol., 9, pp. 237-264, (1993)
[6]  
Fisher W.S., Dinuzzo A.R., Agglutination of bacteria and erythrocytes by serum from six species of marine molluscs, J. Invert. Pathol., 57, pp. 380-394, (1991)
[7]  
Goto R., Muramoto K., Yamazaki M., Et al., Purification and characterization of an agglutinin of the soft coral Sinularia species, Developmental and Comparative Immunology, 16, pp. 9-17, (1992)
[8]  
Hudgin R.L., Ashwell G., Studies on the role of glycosyltransferases in the hepatic binding of asialoglycoproteins, J. Biol. Chem., 249, pp. 7269-7272, (1974)
[9]  
Lowry P.H., Rosebrough N.J., Farr L., Et al., Protein measurement with the folin-phenol reagent, J. Biol. Chem., 193, pp. 256-275, (1951)
[10]  
Matsui T., D-galactoside specific lectins from coelomocytes of the echiuran, Urechis unicinctus, Biol. Bull., 166, pp. 178-188, (1984)