Effects of seasonal ambient heat stress on expression of microRNAs in the mammary gland of Holstein cows

被引:0
作者
Caiyun Fan
Ruiting Hu
Haixiao Fan
Ying Yang
Bin Gong
Shaoze Zhang
Jianping Ding
Yanjing Su
Zhao Zhuo
Jianbo Cheng
机构
[1] Anhui Agricultural University,College of Animal Science and Technology
[2] Bright Farming Co.,undefined
[3] Ltd.,undefined
来源
International Journal of Biometeorology | 2021年 / 65卷
关键词
Heat stress; miRNA; Dairy cows; Mammary gland; MAPK pathway;
D O I
暂无
中图分类号
学科分类号
摘要
This study was conducted to assess the link of miRNA expressions in cow’s mammary gland undergoing heat stress. Twelve Holstein cows were allocated either to undergo heat stress (HS) or remain in a thermoneutral environment (non-heat stress, NS), respectively. The experiment with HS cows was carried out in August, and the experiment with NS cows was done in November. After a month, three cows from each group were slaughtered, and mammary gland samples were obtained, and then miRNA were extracted from the samples for later sequencing. From the miRNA-seq, we obtained a total of 124 differentially expressed miRNAs in HS and NS cows’ mammary gland. The differentially expressed miRNA could be predicted to influence multiple target genes. The target interleukin-1 (IL-1), which play a role in regulating the function of mammary gland in dairy cows, could be affected by bta-let-7c, bta-let-7e, bta-miR-181d, bta-miR-452, and bta-miR-31. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that mitogen-activated protein kinase (MAPK) pathway plays an important role in the mammary glands of dairy cows and bta-miR-25 and bta-miR-382 may influence MAPK pathway through c-Jun N-terminal kinase (JNK) gene to affect the function of mammary gland in HS cows. In conclusion, this study characterized expression profile of miRNAs in the Holstein cows’ mammary gland under summer heat stress or not. We observed miRNA expression during heat stress, which was significantly different from non-heat stress states. A comprehensive analysis of the miRNA’s expression will be helpful to further study the link of miRNAs with mechanisms regulating heat stress in the cow mammary gland.
引用
收藏
页码:235 / 246
页数:11
相关论文
共 50 条
  • [21] Milk production and composition and metabolic alterations in the mammary gland of heat-stressed lactating dairy cows
    FAN Cai-yun
    SU Di
    TIAN He
    HU Rui-ting
    RAN Lei
    YANG Ying
    SU Yan-jing
    CHENG Jian-bo
    Journal of Integrative Agriculture, 2019, 18 (12) : 2844 - 2853
  • [22] Milk production and composition and metabolic alterations in the mammary gland of heat-stressed lactating dairy cows
    Fan Cai-yun
    Su Di
    Tian He
    Hu Rui-ting
    Ran Lei
    Yang Ying
    Su Yan-jing
    Cheng Jian-bo
    JOURNAL OF INTEGRATIVE AGRICULTURE, 2019, 18 (12) : 2844 - 2853
  • [23] Proteomic analysis of the effects of lutein on mammary gland metabolism in dairy cows
    Wang, Caihong
    Wang, Chong
    Liu, Jianxin
    Liu, Hongyun
    JOURNAL OF DAIRY RESEARCH, 2018, 85 (02) : 152 - 156
  • [24] Effects of heat stress abatement on systemic and mammary inflammation in lactating dairy cows
    Chen, Y-C.
    Rivas, R. M. Orellana
    Marins, T. N.
    Melo, Victor H. L. R.
    Wang, Z.
    Garrick, M.
    Gao, J.
    Liu, H.
    Bernard, J. K.
    Melendez, P.
    Tao, S.
    JOURNAL OF DAIRY SCIENCE, 2023, 106 (11) : 8017 - 8032
  • [25] Heat stress has divergent effects on the milk microbiota of Holstein and Brown Swiss cows
    Ceciliani, F.
    Maggiolino, A.
    Biscarini, F.
    Dadi, Y.
    De Matos, L.
    Cremonesi, P.
    Landi, V.
    De Palo, P.
    Lecchi, C.
    JOURNAL OF DAIRY SCIENCE, 2024, 107 (12) : 11639 - 11654
  • [26] Postabsorptive carbohydrate adaptations to heat stress and monensin supplementation in lactating Holstein cows
    Baumgard, L. H.
    Wheelock, J. B.
    Sanders, S. R.
    Moore, C. E.
    Green, H. B.
    Waldron, M. R.
    Rhoads, R. P.
    JOURNAL OF DAIRY SCIENCE, 2011, 94 (11) : 5620 - 5633
  • [27] Optimization of Pulsation Rate of the Milking System for the Mammary Gland Remodeling during Involution in Thai Crossbred Holstein Cows
    Tiantong, A.
    Sasiwimonrit, K.
    Saengwong, S.
    Inyawilert, W.
    Chaokaur, A.
    Chen, S. E.
    TROPICAL ANIMAL SCIENCE JOURNAL, 2021, 44 (01) : 32 - 38
  • [28] Effects of heat stress and nutrition on lactating Holstein cows: II. Aspects of hepatic growth hormone responsiveness
    Rhoads, M. L.
    Kim, J. W.
    Collier, R. J.
    Crooker, B. A.
    Boisclair, Y. R.
    Baumgard, L. H.
    Rhoads, R. P.
    JOURNAL OF DAIRY SCIENCE, 2010, 93 (01) : 170 - 179
  • [29] Identification and bioinformatics analysis of microRNAs associated with stress and immune response in serum of heat-stressed and normal Holstein cows
    Yue Zheng
    Kun-lin Chen
    Xiao-min Zheng
    Hui-xia Li
    Gen-lin Wang
    Cell Stress and Chaperones, 2014, 19 : 973 - 981
  • [30] Oxidative stress, NF-κB signaling, NLRP3 inflammasome, and caspase apoptotic pathways are activated in mammary gland of ketotic Holstein cows
    Sun, Xudong
    Tang, Yan
    Jiang, Chunhui
    Luo, Shengbin
    Jia, Hongdou
    Xu, Qiushi
    Zhao, Chenxu
    Liang, Yusheng
    Cao, Zhijun
    Shao, Guang
    Loor, Juan J.
    Xu, Chuang
    JOURNAL OF DAIRY SCIENCE, 2021, 104 (01) : 849 - 861