Application of droplet digital PCR for the detection of vector copy number in clinical CAR/TCR T cell products

被引:0
|
作者
Alex Lu
Hui Liu
Rongye Shi
Yihua Cai
Jinxia Ma
Lipei Shao
Victor Rong
Nikolaos Gkitsas
Hong Lei
Steven L. Highfill
Sandhya Panch
David F. Stroncek
Ping Jin
机构
[1] NIH Clinical Center,Center for Cellular Engineering, Department of Transfusion Medicine and Cellular Engineering
关键词
Droplet digital PCR; Vector copy number; Genetically engineered T cells; Gene therapy; Chimeric antigen receptor (CAR) T cells; T Cell Receptor (TCR)-engineered T cells; Cellular cancer immunotherapy;
D O I
暂无
中图分类号
学科分类号
摘要
引用
收藏
相关论文
共 50 条
  • [41] High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number
    Hindson, Benjamin J.
    Ness, Kevin D.
    Masquelier, Donald A.
    Belgrader, Phillip
    Heredia, Nicholas J.
    Makarewicz, Anthony J.
    Bright, Isaac J.
    Lucero, Michael Y.
    Hiddessen, Amy L.
    Legler, Tina C.
    Kitano, Tyler K.
    Hodel, Michael R.
    Petersen, Jonathan F.
    Wyatt, Paul W.
    Steenblock, Erin R.
    Shah, Pallavi H.
    Bousse, Luc J.
    Troup, Camille B.
    Mellen, Jeffrey C.
    Wittmann, Dean K.
    Erndt, Nicholas G.
    Cauley, Thomas H.
    Koehler, Ryan T.
    So, Austin P.
    Dube, Simant
    Rose, Klint A.
    Montesclaros, Luz
    Wang, Shenglong
    Stumbo, David P.
    Hodges, Shawn P.
    Romine, Steven
    Milanovich, Fred P.
    White, Helen E.
    Regan, John F.
    Karlin-Neumann, George A.
    Hindson, Christopher M.
    Saxonov, Serge
    Colston, Bill W.
    ANALYTICAL CHEMISTRY, 2011, 83 (22) : 8604 - 8610
  • [42] Copy Number Variation and Rearrangements Assessment in Cancer: Comparison of Droplet Digital PCR with the Current Approaches
    Cusenza, Vincenza Ylenia
    Bisagni, Alessandra
    Rinaldini, Monia
    Cattani, Chiara
    Frazzi, Raffaele
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (09)
  • [43] Decreased mitochondrial DNA copy number in children with cerebral palsy quantified by droplet digital PCR
    Lu, Bichao
    Zeng, Fanyong
    Xing, Wen
    Liang, Lin
    Huo, Jianbo
    Tan, Chianru
    Zhu, Lingxiang
    Liu, Zhizhong
    CLINICA CHIMICA ACTA, 2020, 503 : 122 - 127
  • [44] Quantification of DNA copy number variations in patients with coronary artery disease by digital droplet PCR
    Sleptsov, A. A.
    Nazarenko, M. S.
    Valiakhmetov, N. R.
    Kazantsev, A. N.
    Barbarash, O. L.
    Puzyrev, V. P.
    EUROPEAN JOURNAL OF HUMAN GENETICS, 2019, 27 : 139 - 139
  • [45] DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials
    Corbisier, Philippe
    Pinheiro, Leonardo
    Mazoua, Stephane
    Kortekaas, Anne-Marie
    Chung, Pui Yan Jenny
    Gerganova, Tsvetelina
    Roebben, Gert
    Emons, Hendrik
    Emslie, Kerry
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2015, 407 (07) : 1831 - 1840
  • [46] DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials
    Philippe Corbisier
    Leonardo Pinheiro
    Stéphane Mazoua
    Anne-Marie Kortekaas
    Pui Yan Jenny Chung
    Tsvetelina Gerganova
    Gert Roebben
    Hendrik Emons
    Kerry Emslie
    Analytical and Bioanalytical Chemistry, 2015, 407 : 1831 - 1840
  • [47] An efficient and accurate droplet digital PCR method for rapid transgene copy number detection and homozygous identification in cotton (Gossypium hirsutum)
    He, Qingfei
    Shang, Xiaoguang
    Tian, Ruiping
    Zhu, Xiefei
    Guo, Wangzhen
    INDUSTRIAL CROPS AND PRODUCTS, 2023, 204
  • [48] Development and Application of Droplet Digital PCR Tools for the Detection of Transgenes in Pastures and Pasture-Based Products
    Giraldo, Paula A.
    Cogan, Noel O., I
    Spangenberg, German C.
    Smith, Kevin F.
    Shinozuka, Hiroshi
    FRONTIERS IN PLANT SCIENCE, 2019, 9
  • [49] Considerations for Developing a Droplet Digital PCR Assay for the Detection of Copy Number Variations in Small, Formalin Fixed, Paraffin Embedded Tissue
    Barney, Rachael E.
    Hughes, Edward G.
    O'Hern, Keegan J.
    Baker, Catherine M.
    Tsongalis, Gregory J.
    Sriharan, Aravindhan
    AMERICAN JOURNAL OF PATHOLOGY, 2020, 190 (12): : S30 - S30
  • [50] Optimization of quantitative real-time polymerase chain reaction (qPCR)-based assessment of vector copy number as safety release criterion for clinical grade CAR T cell products
    Kunz, A.
    Gern, U.
    Schmitt, A.
    Neuber, B.
    Wang, L.
    Hueckelhoven-Krauss, A.
    Michels, B.
    Sellner, L.
    Hofmann, S.
    Mueller-Tidow, C.
    Dreger, P.
    Schmitt, M.
    Schubert, M. -L.
    ONCOLOGY RESEARCH AND TREATMENT, 2019, 42 : 238 - 239