Imaging real-time HIV-1 virion fusion with FRET-based biosensors

被引：0
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作者：
Daniel M. Jones
Sergi Padilla-Parra
机构：
[1] University of Oxford,Division of Structural Biology
[2] The Henry Wellcome Building for Genomic Medicine,undefined
[3] Cellular Imaging Core,undefined
[4] Wellcome Trust Centre for Human Genetics,undefined
[5] University of Oxford,undefined
来源：
Scientific Reports | / 5卷
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摘要：
We have produced a novel, simple and rapid method utilising genetically encodable FRET-based biosensors to permit the detection of HIV-1 virion fusion in living cells. These biosensors show high sensitivity both spatially and temporally and allow the real-time recovery of HIV-1 fusion kinetics in both single cells and cell populations simultaneously.
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