Ca2+ Uptake Through Voltage-gated L-type Ca2+ Channels by Polarized Enterocytes from Atlantic Cod Gadus morhua

被引:0
|
作者
D. Larsson
T. Lundgren
K. Sundell
机构
[1] Fish Endocrinology Laboratory,
[2] Department of Zoophysiology,undefined
[3] Göteborg University,undefined
[4] Box 463,undefined
[5] SE-405 30 Göteborg,undefined
[6] Sweden,undefined
[7] Department of Oral Biochemistry,undefined
[8] Göteborg University,undefined
[9] Sweden,undefined
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关键词
Key words: BAY K-8644 — nifedipine — (-)-STBodipy —ω-conotoxin — intestine — teleost;
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摘要
The presence and localization of voltage-gated Ca2+ channels of L-type were investigated in intestinal cells of the Atlantic cod. Enterocytes were loaded with the fluorescent Ca2+ probe, fure-2/AM and changes in intracellular Ca2+ concentrations ([Ca2+]i) were measured, in cell suspensions, in the presence of high potassium levels (100 mm), BAY K-8644 (5 μm), nifedipine (5 μm) or ω-conotoxin (1 μm). L-type Ca2+ channels were visualized on intestinal sections using the fluorescent dihydropyridine (-)-STBodipy.
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页码:229 / 237
页数:8
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