Protective effect of coumarin-pi against t-BHP-induced hepatotoxicity by upregulating antioxidant enzymes via enhanced Nrf2 signaling

被引:0
作者
Jinxiu Zhang
Haiyan Feng
Jianhua Lv
Liqiang Zhao
Junxia Zhao
Li-an Wang
机构
[1] Hebei Normal University,College of Life Science
[2] Jilin Agricultural University,Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education
[3] Hebei Medical University,The Basic Medical College
来源
Molecular and Cellular Biochemistry | 2020年 / 475卷
关键词
Coumarin-pi; HepG2 cells; Oxidative stress; Nrf2; Transcriptome sequencing;
D O I
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学科分类号
摘要
Coumarin-pi, a new coumarin derivative isolated from the mushroom Paxillus involutus, has antioxidative activity, but the underlying mechanism against intracellular oxidative stress is still unclear. This study investigated its cytoprotective effects and the antioxidative mechanism in tert-butyl hydroperoxide (t-BHP)-induced HepG2 cells. The results demonstrated that coumarin-pi suppressed t-BHP-stimulated cytotoxicity, cell apoptosis, and generation of reactive oxygen species (ROS). Additionally, coumarin-pi promoted nuclear factor erythroid 2-related factor 2 (Nrf2) expression and upregulated the protein expression of antioxidantenzymes, including heme oxygenase-1 (HO-1), NAD(P)H: quinone oxidase (NQO1), glutamyl cysteine ligase catalytic subunit (GCLC), and glutamate-cysteine ligase regulatory subunit (GCLM). After coumarin-pi treatment, transcriptome sequencing and bioinformatic analysis revealed that 256 genes were differentially expressed; interestingly, only 20 genes were downregulated, and the rest of the genes were upregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation were used to identify changes in metabolic pathways. Collectively, the results presented in this study indicate that coumarin-pi has a protective effect against t-BHP-induced cellular damage and oxidative stress.
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页码:277 / 283
页数:6
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