RETRACTED ARTICLE: Coexpression of HSP47 Gene and Type I and Type III Collagen Genes in LPS-Induced Pulmonary Fibrosis in Rats

被引:0
作者
Satoshi Hagiwara
Hideo Iwasaka
Shigekiyo Matsumoto
Takayuki Noguchi
Hidekatsu Yoshioka
机构
[1] Oita University Faculty of Medicine,Department of Brain and Nerve Science, Anesthesiology
[2] Oita University Faculty of Medicine,Department of Anatomy, Biology and Medicine, Biochemistry
来源
Lung | 2007年 / 185卷
关键词
Acute respiratory distress syndrome (ARDS); Collagen; Heat shock protein 47; Lipopolysaccharide; Pulmonary fibrosis;
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摘要
Diffuse alveolar damage is the histopathologic hallmark of acute respiratory distress syndrome (ARDS). A significant proportion of ARDS survivors have residual pulmonary fibrosis and compromised pulmonary function. On the other hand, heat shock protein 47 (HSP47) is a collagen-binding stress protein that is assumed to act as a collagen-specific molecular chaperone during the biosynthesis and secretion of procollagen in living cells. The synthesis of HSP47 has been reported to correlate with that of collagen in several cell lines. We examined the expression of HSP47 mRNA and protein during the progression of lipopolysaccharide (LPS)-induced ARDS in rat lung. Male Wistar rats were randomly divided into two groups: a control group with instillation of 0.9% NaCl solution alone, and a LPS group with instillation of LPS dissolved in 0.9% NaCl solution (10 mg/kg). Histologic changes thereafter appeared in the LPS-treated rats. Northern blot analysis revealed the expression of HSP47 mRNA to be markedly induced during the progression of lung damage in parallel with type I and type III collagen mRNA. These results suggest that the upregulation of HSP47 and collagen may play an important role in the fibrotic process of LPS-induced ARDS lung.
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页码:31 / 37
页数:6
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