Synthesis of alkaline protease by catabolite repression- resistant Thermoactinomyces sp. E79 mutant

被引：0
作者：
K. Sunitha
Young-Seo Park
Tae-Kwang Oh
Jung-Kee Lee
机构：
[1] Korea Research Institute of Bioscience & Biotechnology,Microbial Enzyme Research Unit
[2] Kyungwon University,Department of Food and Bioengineering
来源：
Biotechnology Letters | 1999年 / 21卷
关键词：
catabolite repression; mutation; glucose transport; protease; Thermoactinomyces;
D O I：
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学科分类号：
摘要：
The production of alkaline protease from Thermoactinomyces sp. E79 was repressed by 0.2% (w/v) glucose in the medium. Catabolite repression-resistant mutant M1 was obtained by combined treatment of UV light and N-methyl- N′-nitro-N-nitrosoguanidine. The glucose uptake studies accomplished by [14C]glucose showed that the mutant has lost its ability for glucose uptake. The protease production by mutant M1 in the enzyme production medium was 62 U/mg, which was twice that of the wild-type strain.
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页码：155 / 158
页数：3
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