Development of 134 novel polynucleotide-repeat microsatellite markers in silver carp (Hypophthalmichthys molitrix)

被引：0
作者：
Wenjie Guo
Xiaomu Yu
Jingou Tong
机构：
[1] Institute of Hydrobiology,State Key Laboratory of Freshwater Ecology and Biotechnology
[2] The Chinese Academy of Sciences,undefined
[3] University of Chinese Academy of Sciences,undefined
来源：
Conservation Genetics Resources | 2013年 / 5卷
关键词：
Silver carp; Microsatellite; Polymorphism; Bighead carp; Cross-species amplification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, we developed 134 novel polymorphic polynucleotide-repeat microsatellite markers for silver carp (Hypophthalmichthys molitrix) and characterized 40 loci for polymorphism and genetic diversity in a test population. The number of alleles per locus ranged from 3 to 19 (mean 9.4), and the average observed and expected heterozygosities were 0.74–0.76, respectively. Thirty-five loci were highly informative (PIC > 0.5) in silver carp population. After Bonferroni adjustments, no pairs of loci showed evidence of significant linkage disequilibrium, and none of the loci significantly deviated from the Hardy-Weinberg equilibrium. All 134 silver carp microsatellite loci could be successfully amplified in bighead carp (Hypophthalmichthys nobilis) with 65 loci being polymorphic, indicating a high transferability of these microsatellites across species. This set of novel highly polymorphic polynucleotide—repeat microsatellites would be powerful tools for population and conservation genetics studies in silver carp and also in its closely-related bighead carp.
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页码：525 / 528
页数：3
相关论文
共 56 条
[1]  
Botstein D(1980)Construction of a genetic linkage map in man using restriction fragment length polymorphisms Am J Hum Genet 32 314-331
[2]  
White RL(1997)Automated sizing of fluorescent-labeled simple sequence repeat (SSR) markers to assay genetic variation in soybean Theor Appl Genet 95 723-733
[3]  
Skolnick M(1992)Microsatellites for linkage analysis of genetic traits Trends Genet 8 288-294
[4]  
Davis RW(2006)SSR primer and SSR taxonomy tree: biome SSR discovery Nucleic Acids Res 34 W656-W659
[5]  
Diwan N(2005)SSR hunter: development of a local searching software for SSR sites Hereditas (Beijing) 5 808-810
[6]  
Cregan PB(2006)Development of microsatellite DNA markers of silver carp ( J Fishery Sci China 13 756-761
[7]  
Hearne CM(2010)) and their application in the determination of genetic diversities of silver carp and bighead carp ( Chin J Fisheries 23 52-59
[8]  
Ghosh S(2000)) Nat Biotechnol 18 233-234
[9]  
Todd JA(1992)The progress of germplasm resources of four major Chinese farmed carps J Fish Biol 40 963-965
[10]  
Jewell E(2004)An economic method for the fluorescent labeling of PCR fragments Mol Ecol Resour 4 535-538
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