Transformation of Phospholipids by Cabbage Phospholipase D in Mixed Micelles Containing 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

被引：0
作者：
V. M. Shnigir
M. A. Kisel'
机构：
[1] National Academy of Sciences of Belarus,Institute of Bioorganic Chemistry
来源：
Applied Biochemistry and Microbiology | 2004年 / 40卷
关键词：
Enzyme; Hydrolysis; Physicochemical Property; Phosphatidylcholine; Transesterification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We compared the activities of cabbage phospholipase D during hydrolysis and transesterification of phosphatidylcholine in mixed micelles of surface-active compounds with various physicochemical properties. Mixed micelles of phospholipids and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (ratio, 1 : 2) were among the best substrates. Hydrolysis and transphosphatidylation were studied in micelles containing 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Mixed micelles of phosphatidylcholine and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate may serve as a new substrate for the measurement of phospholipase D activity and preparation of phospholipids using this enzyme.
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页码：225 / 230
页数：5
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