In vitro induction of multiple shoots and plant regeneration in Vigna

被引：22
作者：
Sen J. [1 ]
Guha-Mukherjee S. [1 ]
机构：
[1] Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University
来源：
In Vitro Cellular & Developmental Biology - Plant | 1998年 / 34卷 / 4期
关键词：
Histology; Multiple shoot formation; Shoot bud regeneration; Vigna sp;
D O I：
10.1007/BF02822733
中图分类号：
学科分类号：
摘要：
Cotyledonary nodes, excised cotyledons, and hypocotyl segments of six varieties of Vigna mungo and V. radiata have been tested for their morphogenic potential on media containing a range of hormonal combinations including benzyladenine, kinetin, thidiazuron (TDZ), and zeatin. Multiple shoots developed on cotyledonary node explants in all varieties tested on basal medium containing cytokinin. Presence of both the cotyledons, either full or half, resulted in a maximum number of shoots produced. Shoot bud regeneration was achieved via meristem formation on excised cotyledons on Murashige-Skoog basal medium with B5 vitamins supplemented with TDZ. Mature plants had normal phenotypes. V. mungo var. PS1 and V. radiata var. Pusa105 were found to be the most responsive varieties for shoot regeneration. The histology of in vitro organogenesis was studied.
引用
收藏
页码：276 / 280
页数：4
相关论文
共 15 条
  • [1] Chandra M., Pal A., Differential response of two cotyledons of Vigna radiata in vitro, Plant Cell Rep., 15, pp. 248-253, (1995)
  • [2] Dineshkumar V., Kirti P.B., Sachan J.K.S., Et al., Picloram induced somatic embryogenesis in chickpea (Cicer arietinum L.), Plant Sci., 109, pp. 207-213, (1995)
  • [3] Eapen S., George L., Ontogeny of somatic embryos of Vigna aconitifolia, Vigna mungo and Vigna radiata, Ann. Bot., 66, pp. 219-226, (1990)
  • [4] Gamborg O.L., Miller R.A., Ojima K., Nutrient requirements of suspension cultures of soybean root cells, Exp. Cell. Res., 15, pp. 151-158, (1968)
  • [5] Gulati A., Jaiwal P.K., Culture conditions affecting plant regeneration from cotyledons of V. radiata (L.) Wilczek, Plant Cell Tissue Organ Cult., 23, pp. 1-7, (1990)
  • [6] Gulati A., Jaiwal P.K., Plant regeneration from cotyledonary node explants of mungbean (Vigna radiata (L.) Wilczek), Plant Cell Rep., 13, pp. 523-527, (1994)
  • [7] Johansen D.A., Plant microtechnique, pp. 126-154, (1940)
  • [8] Karthikeyan A.S., Sarma K.S., Veluthambi K., Agrobacterium tumefaciens mediated transformation of Vigna mungo (L). Hepper, Plant Cell Rep., 15, pp. 328-331, (1996)
  • [9] Mathews H., Morphogenetic responses from in vitro cultured seedlings in mung bean (V. radiata L. Wilczek), Plant Cell Tissue Organ Cult., 11, pp. 233-240, (1987)
  • [10] Murashige T., Skoog F., A revised medium for rapid bioassays with tobacco tissue cultures, Physiol. Plant., 15, pp. 473-493, (1962)
12