Analysis of nicastrin gene phylogeny and expression in zebrafish

被引：0
作者：
Anne Lim
Seyyed Hani Moussavi Nik
Esmaeil Ebrahimie
Michael Lardelli
机构：
[1] The University of Adelaide,Alzheimer’s disease Genetics Laboratory, School of Molecular and Biomedical Science
[2] The University of Adelaide,Alzheimer’s disease Genetics Laboratory, Department of Genetics and Evolution
来源：
Development Genes and Evolution | 2015年 / 225卷
关键词：
Zebrafish; Nicastrin; Quantitative real-time PCR; In situ hybridization;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
NICASTRIN is a component of the aspartyl protease γ-secretase complex which is involved in intramembranous cleavage of type I transmembrane proteins, notably the Notch receptor proteins and the AMYLOID BETA A4 PRECURSOR PROTEIN (APP). This study aimed to characterize the orthologue of the human NICASTRIN (NCSTN) gene in zebrafish, an advantageous model organism for the study of human disease. Zebrafish Nicastrin protein was predicted to possess the conserved glutamate 333 residue and DYIGS motif of human NCSTN that are important for substrate recognition/processing in γ-secretase. Quantitative real-time RT-PCR revealed the profile of relative zebrafish nicastrin (ncstn) transcript levels in embryos at different times during development and in adult tissues. The analysis of synteny conservation revealed local rearrangements of ncstn and another gene, mpz, relative to copa, and pex19. In situ hybridization showed higher relative levels of ncstn transcripts in the developing brain and otic vesicles of embryos at 24 and 48 h post fertilization, respectively. Our observations are consistent with a role for Ncstn protein in Notch signaling within the proliferative ventricular zone of the developing central nervous system.
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页码：171 / 178
页数：7
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