Comprehensive genomic access to vector integration in clinical gene therapy

被引:0
作者
Richard Gabriel
Ralph Eckenberg
Anna Paruzynski
Cynthia C Bartholomae
Ali Nowrouzi
Anne Arens
Steven J Howe
Alessandra Recchia
Claudia Cattoglio
Wei Wang
Katrin Faber
Kerstin Schwarzwaelder
Romy Kirsten
Annette Deichmann
Claudia R Ball
Kamaljit S Balaggan
Rafael J Yáñez-Muñoz
Robin R Ali
H Bobby Gaspar
Luca Biasco
Alessandro Aiuti
Daniela Cesana
Eugenio Montini
Luigi Naldini
Odile Cohen-Haguenauer
Fulvio Mavilio
Adrian J Thrasher
Hanno Glimm
Christof von Kalle
William Saurin
Manfred Schmidt
机构
[1] National Center for Tumor Diseases and German Cancer Research Center,Department of Translational Oncology
[2] Genomining,Department of Biomedical Sciences
[3] Genomics and Proteomics Core Facilities,Department of Clinical Immunology
[4] German Cancer Research Center,Department of Public Health and Cell Biology
[5] Molecular Immunology Unit,Department of Clinical Oncology
[6] Institute of Child Health,undefined
[7] University College,undefined
[8] University of Modena and Reggio Emilia,undefined
[9] Cancer Immunotherapy and Gene Therapy Program,undefined
[10] Instituto Scientifico H. San Raffaele,undefined
[11] Institute of Ophthalmology,undefined
[12] University College London,undefined
[13] School of Biological Sciences,undefined
[14] Royal Holloway–University of London,undefined
[15] Great Ormond Street Hospital NHS Trust,undefined
[16] San Raffaele Telethon Institute for Gene Therapy,undefined
[17] University of Rome Tor Vergata,undefined
[18] Laboratoire de Biotechnologie et Pharmacologie Génétique Appliquées,undefined
[19] Ecole Normale Supérieure de Cachan,undefined
[20] Oncogenetics,undefined
[21] Hôpital Saint-Louis,undefined
[22] Molecular and Gene Therapy Program,undefined
[23] Cincinnati Children's Research Foundation,undefined
来源
Nature Medicine | 2009年 / 15卷
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摘要
Adverse events stemming from the use of retroviral vectors in humans has prompted the search for methods predicting the fate and biological consequences of gene-modified cells after vector insertion. Methods of integration site analysis, such as linear amplification-mediated PCR (LAM-PCR), rely on use of restriction enzymes and identify only a fraction of all genomic integrants. This report describes a non–restriction enzyme–based LAM-PCR technique that provides comprehensive, unbiased integration site analysis.
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页码:1431 / 1436
页数:5
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