Protamine Sulfate Enhances the Transduction Efficiency of Recombinant Adeno-Associated Virus-Mediated Gene Delivery

被引:0
作者
Ya-Wun Yang
Yuan-Chiao Hsieh
机构
[1] National Taiwan University,School of Pharmacy, College of Medicine
来源
Pharmaceutical Research | 2001年 / 18卷
关键词
recombinant adeno-associated virus; protamine sulfate; gene delivery; hepatoma cells; zeta potential;
D O I
暂无
中图分类号
学科分类号
摘要
Purpose. The purpose of this study was to evaluate glucose responsiveness in HepG2 human hepatoma cells transduced by a recombinant adeno-associated virus (rAAV) vector containing the insulin gene promoter, and to investigate the effect of protamine sulfate on rAAV-mediated gene delivery.
引用
收藏
页码:922 / 927
页数:5
相关论文
共 97 条
[11]  
Zhu X. D.(1999)Towards gene therapy of diabetes mellitus Mol. Medicine Today 5 165-171
[12]  
Hunter L.(1990)Modulation of glucose-induced insulin secretion from a rat clonal β-cell line Endocrinol. 127 2779-2788
[13]  
Laughlin C. A.(1995)Glucose responsiveness of a reporter gene transduced into hepatocytic cells using a retroviral vector FEBS Lett. 365 223-226
[14]  
McLaughlin S.(1998)Regulatable production of insulin from primary-cultured hepatocytes: Insulin production is up-regulated by glucagon and cAMP and down-regulated by insulin Gene Ther. 5 888-895
[15]  
Muzyczka N.(1996)Synthesis and processing of genetically modified human proinsulin by rat myoblast in primary cultures Hum. Gene Ther. 7 71-78
[16]  
Rocchi M.(1997)Gene therapy of diabetes: Glucose-stimulated insulin secretion in a human hepatoma cell line (HEPG2ins/g) Gene Ther. 4 1202-1215
[17]  
Berns K. I.(2000)Glucose regulated production of human insulin in rat hepatocytes Gene Ther. 7 205-214
[18]  
Berns K. I.(2000)Engineering of a glucose-responsive surrogate cell for insulin replacement therapy of experimental insulin-dependent diabetes Hum. Gene Ther. 11 403-414
[19]  
Goodman S.(2000)Glucose-responsive gene delivery in pancreatic islet cells via recombinant adeno-associated viral vectors Pharm. Res. 17 1056-1061
[20]  
Xiao X.(1999)Adeno-associated virus as a gene delivery vector for liver cells J. Hepatol. 31 385-385