Detection of low-abundant novel transcripts in mouse hematopoietic stem cells

被引：0
作者：
Hyeng-Soo Kim
Junmo Hwang
Young-Hun Kim
Seonggon Kim
Jae-Woong Lee
Hyung-Sik Kang
Kil Soo Kim
Ji-Hong Ha
Jin Woong Chung
Kyu-Tae Chang
Zae Young Ryoo
Sanggyu Lee
机构：
[1] Kyungpook National University,School of Life Science and Biotechnology
[2] Chonnam National University,School of Biological Sciences and Technology, Hormone Research Institute
[3] Kyungpook National University,Department of Veterinary Medicine, College of Veterinary Medicine
[4] Dong-A University,Department of Biological Science, College of Natural Sciences
[5] Korea Research Institute of Bioscience and Biotechnology,National Primate Research Center
来源：
Molecular Genetics and Genomics | 2009年 / 282卷
关键词：
Transcriptome; Hematopoietic stem cell; SAGE; Gene expression; Gr-1 myeloid progenitor cell;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Gene expression profiles of hematopoietic stem cells (HSCs) provide clues for understanding molecular mechanisms of HSC behavior, including self-renewal and differentiation. We took advantage of serial analysis of gene expression (SAGE) to identify medium- and low-abundant transcripts expressed in HSCs/hematopoietic progenitor cells (HPCs). Among a total of 31,380 unique transcripts, 17,326 (55%) correspond to known genes and, 14,054 (45%) are low-copy transcripts that have no matches to currently known genes. Among the former class, 3,899 (23%) were alternatively spliced transcripts and 3,754 (22%) represent anti-sense transcripts from known genes. Mapping of the SAGE tags to the mouse genome showed that differences in gene expression exist among chromosomes. In addition, comparison of the HSCs/HPCs SAGE data to that of myeloid progenitor cells revealed that massive genetic reprogramming occurs in hematopoietic cell differentiation. Our results demonstrate a previously unrecognized complexity of gene expression in HSCs/HPCs, and indicate the need for further efforts to fully identify and characterize the transcripts expressed in this cell type.
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页码：363 / 370
页数：7
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