Fluorescent In Situ Hybridization Analysis of Philadelphia Chromosome-Negative Chronic Myeloid Leukemia with the bcr/abl Fusion Gene

被引：0

作者：

Fumihiko Monma

Kazuhiro Nishii

Shunji Yamamori

Noboru Hosokai

Takahiro Nakazaki

Felipe Lorenzo V

Eiji Usui

Miho Sakakura

Hiroyuki Miyashita

Atsushi Fujieda

Kohshi Ohishi

Naoyuki Katayama

Hiroshi Shiku

机构：

[1] Mie University School of Medicine,Second Department of Internal Medicine

[2] Mitsubishi Kagaku Bio-Clinical Laboratories,Internal Medicine

[3] Inc,undefined

[4] Takeuchi Hospital,undefined

来源：

International Journal of Hematology | 2004年 / 80卷

关键词：

Philadelphia chromosome; Masked Ph; t(9); t(22); CML; Imatinib; Fish; Ph-negative CML;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This report describes a patient with Philadelphia chromosome-negative (Ph-) but bcr/abl fusion gene-positive chronic myeloid leukemia (CML) and a molecular analysis of the mechanisms behind the Ph- status. Spectral karyotyping-fluorescent in situ hybridization (SKY-FISH) analysis showed no abnormal translocation; however, a bcr/abl fusion gene was detected by reverse transcriptase-polymerase chain reaction analysis. FISH analysis showed that signals from the 9q and 22q subtelomere probes were detected on the der(9) and der(22) chromosomes, respectively. On the other hand, FISH analysis of the abl and bcr genes with dual fusion probes, which can detect the bcr/abl fusion gene on both the der(9) and der(22) chromosomes, showed the signal for bcr/abl fusion on the der(22) chromosome but not on the der(9) chromosome.These results indicate that insertion of the abl gene into the bcr region on the der(22) chromosome or retranslocation between the der(9) chromosome and the der(22) chromosome may have caused the Ph- CML in this case.

引用

页码：155 / 158

页数：3

共 62 条

[1]

Cortes JE(1995)Philadelphia chromosomenegative chronic myelogenous leukemia with rearrangement of the breakpoint cluster region: long term follow-up results Cancer. 75 464-470

[2]

Talpaz M(1996)Duplication of chromosome 9 carrying a BCR/ABL chimeric gene in Philadelphia chromosome negative chronic myeloid leukemia Cancer Genet Cytogenet. 89 166-169

[3]

Beran M(1986)Rearrangement in the breakpoint cluster region and the clinical course in Philadelphianegative chronic myelogenous leukemia Ann Intern Med. 105 673-679

[4]

Takahashi N(1989)Cytogenetic and molecular analysis in Philadelphia negative CML Blood. 73 1038-1044

[5]

Miura I(2003)Chronic myeloid leukemia: advances in biology and new approaches to treatment N Engl J Med. 349 1451-1464

[6]

Ohshima A(1988)The karyotype of Philadelphia chromosome-negative, Cancer Genet Cytogenet. 35 223-229

[7]

Kurzrock R(1994) rearrangement-positive chronic myeloid leukemia Cancer Genet Cytogenet. 78 153-159

[8]

Blick MD(1993)Complex translocation of the Ph chromosome and Ph negative CML arise from similar mechanisms, as evidenced by FISH analysis Genes Chromosomes Cancer. 8 237-245

[9]

Talpaz M(1989)Translocation of Hum Genet. 83 115-118

[10]

van der Plas DC(1990) to chromosome 9: a new cytogenetic variant detected by FISH in two Phnegative, BCR-positive patients with chronic myeloid leukemia Blood. 76 1812-1818

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