A simple structured model for recombinant IDShr protein production in Pichia pastoris

被引：0
作者：
Diego Fernando Mendoza Muñoz
Néstor Ariel Algecira Enciso
Henry Córdoba Ruiz
Luis Alejandro Barrera Avellaneda
机构：
[1] National University of Colombia,Chemical Engineering Department
[2] Javeriana Pontificia University,Institute of Inborn Errors of Metabolism (IEIM)
来源：
Biotechnology Letters | 2008年 / 30卷
关键词：
Kinetic model; Parameter Estimation; Hunter syndrome;
D O I：
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学科分类号：
摘要：
A simple structured model is proposed for the methanol production phase of the iduronate 2-sulphate sulfatase recombinant enzyme (IDShr) in Pichia patoris Mut+. The model is mainly focused in oxidative stress phenomenon due to methanol consumption and based on extracellular experimental information and the basic knowledge of methanol metabolism in Pichia pastoris yeast (P. pastoris). The model’s prediction shows a reasonable accuracy as compared with the experimental data. Likewise, it was proved that this model is able to simulate the production of other recombinant protein in P. pastoris.
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页码：1727 / 1734
页数：7
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共 91 条
  • [1] Brady RO(2006)Enzyme replacement for lysosomal diseases Ann Rev Med 57 283-296
  • [2] Cereghino JL(2000)Heterologous protein expression in the methylotrophic yeast FEMS Microb Rev 24 45-66
  • [3] Cregg JM(2005)Expression of heterologous proteins in J Mol Recognit 18 119-138
  • [4] Daly R(2005): a useful experimental tool in protein engineering and production Ann Rev Genomics Hum Genet 6 355-379
  • [5] Hearn TW(2003)Sulphatases and human disease J Biotechnol 102 281-290
  • [6] Diez-Roux G(2002)Assessing viability and cell-associated product recombinant producing Bioprocess Biosyst Eng 24 385-393
  • [7] Ballabio A(2002) with flow cytometry J Biotechnol 102 45-53
  • [8] Hohenblum H(2007)Modeling of growth and energy metabolism of Process Biochem 42 828-833
  • [9] Borth N(2006) producing a fusion protein Enz Microb Technol 39 936-944
  • [10] Mattanovich D(2007)Analysis and control of proteolysis of a fusion protein in J Biotechnol 130 236-246
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