Expression of inhibin/activin subunits alpha (-α), BetaA (-βA), and BetaB (-βB) in placental tissue of normal, preeclamptic, and HELLP pregnancies

被引:0
作者
I. Mylonas
B. Schiessl
U. Jeschke
J. Vogl
A. Makrigiannakis
C. Kuhn
S. Schulze
F. Kainer
K. Friese
机构
[1] University of Newcastle upon Tyne,School of Surgical and Reproductive Sciences (Obstetrics & Gynecology)
[2] University of Crete,Department of Obstetrics and Gynecology
[3] Ludwig-Maximilians-University Munich,1st Department of Obstetrics and Gynecology
来源
Endocrine Pathology | 2006年 / 17卷
关键词
Inhibin/activin subunits; inhibin-α; inhibin-βA; inhibin-βB; preeclampsia; HELLP; extravillous trophoblast; syncytotrophoblast;
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摘要
During human pregnancy the placenta produces a variety of proteins for the establishment of the fetoplacental unit, including inhibins and activins. Inhibins are dimeric glycoproteins, composed of an α-subunit and one of two possible β-subunits (βA or βB). Aims of the present study were (a) the determination of the frequency and tissue distribution patterns of the inhibin/activin subunits in human placental tissue of normal pregnancies and pregnancies complicated with preeclampsia and HELLP syndrome (hemolysis, elevated liver enzymes, low platelets) and (b) the assessment of a combined expression of inhibin-α- and both β-subunits (βA- and βB-subunits) using double immunofluorescence technique. A significant lower expression of the inhibin-α subunit in preeclamptic and HELLP placental tissue compared to normal pregnancies was observed, while the inhibin-α immunostaining was significantly upregulated in syncytotrophoblast. Additionally, we demonstrated a significant down-regulation of inhibin-βB subunit in extravillous trophoblast cells between normal and preeclamptic compared to HELLP placental tissue, while inhibin-βA-subunit was significantly higher in preeclamptic syncytotrophoblast cells. A colocalization of inhibin-α and the beta-subunits could be demonstrated, suggesting a production and secretion of intact inhibin A and inhibin B. Therefore, inhibin A and activin A might be useful markers in preeclampsia. Valuable parameters in HELLP syndrome could be inhibin A, rather than inhibin B, and activin B. Furthermore, the lower βB-subunit production in extravillous trophoblast cells demonstrates that this subunit might have an important role in the pathogenesis of HELLP syndrome. Additionally, the higher production of the βA-subunit in syncytotrophoblast cells suggest a higher production of activin A rather than inhibin A in preeclampsia that might be utilized as a marker of placental function.
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页码:19 / 33
页数:14
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