Quantitative Analysis of the Migration and Accumulation of Bacillus subtilis in Asparagus officinalis

被引：0
作者：
Bian-Qing Hao
Li-Ping Ma
Xiong-Wu Qiao
机构：
[1] Shanxi University,Institute of Loess Plateau
[2] Shanxi Academy of Agricultural Sciences,Institute of Agro
来源：
Current Microbiology | 2015年 / 71卷
关键词：
Green Fluorescent Protein; Biological Control Agent; Xylem Vessel; Trichoderma Harzianum; Xanthomonas Campestris;
D O I：
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学科分类号：
摘要：
Bacillus subtilis B96-II is a broad-spectrum biological control strain. It effectively suppresses soil-borne fungal diseases in vegetables. A green fluorescence protein (GFP) was expressed in B96-II to detect migration of B96-II into the root and stem of asparagus. The GFP-tagged B96-II (B96-II-GFP) strain exhibited bright green fluorescence under a fluorescence microscope. GFP was stable and had no apparent effects on the growth of the strain. Asparagus plants were planted in the soil inoculated with B96-II-GFP. Our results showed that B96-II-GFP was detected in both the root and stem 15, 30, and 45 days after the asparagus seedlings were planted. B96-II-GFP was also detected in leaves but at a lower concentration. The highest concentration was detected in 15 days, and the number of bacteria decreased subsequently irrespective of duration of growth or sampling period. The highest concentration of B96-II-GFP was present in the root base suggesting that the root base served as the hub of bacterial migration from the soil to the stem.
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页码：357 / 362
页数：5
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